On the Similarity Between the TRNAs of Organelles and Prokaryotes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1971 Dec 1

PMID 4257127

Citations 7

Authors

S A Fairfield

W E Barnett

Affiliations

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Abstract

Fluorescence studies with organelle transfer RNAs separated from their cytoplasmic counterparts revealed that phenylalanine tRNA from Euglena chloroplasts or Neurospora mitochondria does not contain a fluorescent "base Y." In contrast, cytoplasmic phenylalanine tRNA from Euglena and cytoplasmic tRNA from Neurospora were found to contain fluorescent bases. The fluorescence-emission spectra of Neurospora cytoplasmic tRNAs and those of the related ascomycete Saccharomyces cerevisiue were observed to be quite different. The results support the generalization that eukaryotic tRNAs contain a fluorescent base, but indicate that their respective organelle tRNAs do not. They also indicate a striking parallelism between organelle and prokaryotic tRNAs.

Citing Articles

Cytokinins in tRNA Obtained from Spinacia oleracea L. Leaves and Isolated Chloroplasts.

Vreman H, Thomas R, Corse J Plant Physiol. 1978; 61(2):296-306.

PMID: 16660279 PMC: 1091851. DOI: 10.1104/pp.61.2.296.

Mitochondrial tyrosyl transfer ribonucleic Acid in soybean seedlings.

Meng R, Vanderhoef L Plant Physiol. 1972; 50(2):298-302.

PMID: 16658160 PMC: 366128. DOI: 10.1104/pp.50.2.298.

A Cytoplasmic Regulatory Mutant of Euglena: Constitutivity for the Light-Inducible Chloroplast Transfer RNAs.

Goins D, Reynolds R, Schiff J, Barnett W Proc Natl Acad Sci U S A. 1973; 70(6):1749-52.

PMID: 16592092 PMC: 433587. DOI: 10.1073/pnas.70.6.1749.

The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.

Hecker L, Egan J, Reynolds R, Nix C, Schiff J, Barnett W Proc Natl Acad Sci U S A. 1974; 71(5):1910-4.

PMID: 4525469 PMC: 388352. DOI: 10.1073/pnas.71.5.1910.

Chromatographic analyses of isoaccepting tRNAs from avian myeloblastosis virus.

Gallagher R, Gallo R J Virol. 1973; 12(3):449-57.

PMID: 4355849 PMC: 356650. DOI: 10.1128/JVI.12.3.449-457.1973.

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