The Metabolic Heterogeneity of Rabbit Ear Cartilage Chondroitin Sulphate

Overview

Journal Biochem J

Specialty Biochemistry

Date 1970 Feb 1

PMID 4244918

Citations 5

Authors

J P Bentley

B Rokosova

Affiliations

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Abstract

The only glycosaminoglycans that can be isolated from the ear cartilage of 2-month-old rabbits are chondroitin 4-sulphate and chondroitin 6-sulphate. These chondroitin sulphates exhibit molecular-weight polydispersity when isolated from tissue by papain digestion. The chondroitin sulphate is metabolically heterogeneous in that radioactive precursors [(14)C]glucose or [(35)S]sulphate are preferentially incorporated into the higher-molecular-weight polymers both in vivo and in vitro. No transfer of radioactivity from the high-molecular-weight chondroitin sulphate to the low-molecular-weight chondroitin sulphate was seen during 15 days in vivo. It is suggested that there are at least two pools of proteoglycan in the tissue. One of these pools is metabolically active whereas the other is not.

Citing Articles

Protein-polysaccharide biosynthesis. Membrane-bound saccharides.

Derge J, Davidson E Biochem J. 1972; 126(1):217-23.

PMID: 5075232 PMC: 1178367. DOI: 10.1042/bj1260217.

[Structure, biosynthesis and heterogeneity of chondroitin sulfate proteins].

KLEINE T Naturwissenschaften. 1972; 59(2):64-71.

PMID: 5015751 DOI: 10.1007/BF00593465.

The glycosaminoglycans of the different layers of bovine articular cartilage in relation to age. II. Incorporation of 35s-sulphate in vitro into different fractions of chondroitin sulphate.

Larsson S, Lemperg R Calcif Tissue Res. 1974; 15(3):253-67.

PMID: 4278319 DOI: 10.1007/BF02059061.

The metabolic heterogeneity of glycosaminoglycans of the different zones of the epiphyseal growth plate and the effect of ethane-1-hydroxy-1, 1-diphosphonate (EHDP) upon glycosaminoglycan synthesis in vivo.

Larsson S Calcif Tissue Res. 1976; 21(2):67-82.

PMID: 825203 DOI: 10.1007/BF02547384.

Calf articular cartilage in organ culture in a chemically defined medium. 2. Concentrations of glycosaminoglycans and [35S]-sulfate incorporation at different oxygen tensions.

Lemperg R, BERGENHOLTZ A, Smith T In Vitro. 1975; 11(5):291-301.

PMID: 126946 DOI: 10.1007/BF02615640.

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