Mammalian Mitochondrial Transfer RNAs: Chromatographic Properties, Size and Origin

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1979 Feb 1

PMID 424302

Citations 7

Authors

L Aujame

K B Freeman

Affiliations

Soon will be listed here.

Abstract

Incubation of isolated rat liver mitochondria with radioactive amino acids resulted in the charging of tRNAs for arginine, asparagine, leucine, lysine, methionine, proline and valine. The aminoacyl-tRNAs were shown to be distinct from their cytosolic counterparts by chromatography on RPC-5. By electrophoresis on urea polyacrylamide slab gels it was found that all these mitochondrial aminoacyl-tRNAs were about 70-76 nucleotides long. The unique mitochondrial asparaginyl- and prolyl-tRNAs, not previously identified in mammalian cells, were shown to hybridize to mtDNA. Mitochondrial leucyl-tRNA separated into 3 peaks on RPC-5 and the first species was shown to be different than a combination of the other two by molecular size and partial RNase T1 digestion patterns. Each was coded by a separate gene on mtDNA as shown by partial additivity of hybridization. Separate genes for mitochondrial tRNAMetm and tRNAMetf, separated by RPC-5 chromatography, were also demonstrated. These results bring to 21 the number of individual tRNAs coded by mammalian mtDNA.

Citing Articles

tRNA genes in rat liver mitochondrial DNA.

Grosskopf R, Feldmann H Curr Genet. 2013; 4(3):191-6.

PMID: 24185992 DOI: 10.1007/BF00420498.

Analysis of a DNA segment from rat liver mitochondria containing the genes for the cytochrome oxidase subunits I, II and III, ATPase subunit 6, and several tRNA genes.

Grosskopf R, Feldmann H Curr Genet. 2013; 4(2):151-8.

PMID: 24185961 DOI: 10.1007/BF00365694.

The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons.

Bilbille Y, Gustilo E, Harris K, Jones C, Lusic H, Kaiser R J Mol Biol. 2010; 406(2):257-74.

PMID: 21168417 PMC: 3662834. DOI: 10.1016/j.jmb.2010.11.042.

Identification of two methionine transfer RNA genes in the maize mitochondrial genome.

Parks T, Dougherty W, Levings C, Timothy D Plant Physiol. 1984; 76(4):1079-82.

PMID: 16663952 PMC: 1064437. DOI: 10.1104/pp.76.4.1079.

Different pattern of codon recognition by mammalian mitochondrial tRNAs.

Barrell B, Anderson S, Bankier A, de Bruijn M, Chen E, Coulson A Proc Natl Acad Sci U S A. 1980; 77(6):3164-6.

PMID: 6932013 PMC: 349574. DOI: 10.1073/pnas.77.6.3164.

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