Fibronectin Expression is Determined by the Genotype of the Transformed Parental Cells in Heterokaryons Between Normal and Transformed Fibroblasts

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1979 Jan 1

PMID 422645

Citations 3

Authors

P Laurila

J WARTIOVAARA

S Stenman

Affiliations

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Abstract

The expression of fibronectin, a cell surface-associated transformation-sensitive glycoprotein, was studied in hetero- and homokaryons of normal and SV40-transformed human fibroblasts. In immunofluorescence, fibroblast homokaryons had an intense surface-associated and intracelluar fibronectin fluorescence similar to that of normal fibroblasts. Transformed cells and their homokaryons had a minimal surface-associated and a weak intracellular fibronectin fluorescence. In heterokaryons formed between transformed and normal fibroblasts, the expression of fibronectin fell within 24 h to the level of the transformed cell homokaryons. The change was detectable already at 3 h after fusion and was gene-dose dependent. These results show that the transformed genotype determines fibronectin expression in the heterokaryons.

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References

Yamada K, Yamada S, Pastan I . Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts. Proc Natl Acad Sci U S A. 1976; 73(4):1217-21. PMC: 430233. DOI: 10.1073/pnas.73.4.1217. View

KOPROWSKI H, Croce C . Tumorigenicity of simian virus 40-transformed human cells and mouse--human hybrids in nude mice. Proc Natl Acad Sci U S A. 1977; 74(3):1142-6. PMC: 430625. DOI: 10.1073/pnas.74.3.1142. View

Miller C, Fuseler J, Brinkley B . Cytoplasmic microtubules in transformed mouse x nontransformed human cell hybrids: correlation with in vitro growth. Cell. 1977; 12(1):319-31. DOI: 10.1016/0092-8674(77)90210-0. View

Hedman K, Vaheri A, WARTIOVAARA J . External fibronectin of cultured human fibroblasts is predominantly a matrix protein. J Cell Biol. 1978; 76(3):748-60. PMC: 2109997. DOI: 10.1083/jcb.76.3.748. View

WARTIOVAARA J, Leivo I, Virtanen I, Vaheri A, Graham C . Appearance of fibronectin during differentiation of mouse teratocarcinoma in vitro. Nature. 1978; 272(5651):355-6. DOI: 10.1038/272355a0. View

Gordon S, Cohn Z . Macrophage-melanoma cell heterokaryons. 3. The activation of macrophage DNA synthesis. Studies with inhibitors of protein synthesis and with synchronized melanoma cells. J Exp Med. 1971; 134(4):935-46. PMC: 2138993. DOI: 10.1084/jem.134.4.935. View

Steplewski Z, Knowles B, KOPROWSKI H . The mechanism of internuclear transmission of SV40-induced complement fixation antigen in heterokaryocytes. Proc Natl Acad Sci U S A. 1968; 59(3):769-76. PMC: 224742. DOI: 10.1073/pnas.59.3.769. View

Weiss M . Further studies on loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells. Proc Natl Acad Sci U S A. 1970; 66(1):79-86. PMC: 286090. DOI: 10.1073/pnas.66.1.79. View

Harris H, Wiener F, Klein G . The analysis of malignancy by cell fusion. 3. Hybrids between diploid fibroblasts and other tumour cells. J Cell Sci. 1971; 8(3):681-92. DOI: 10.1242/jcs.8.3.681. View

10.

RINGERTZ N, Carlsson S, Ege T, Bolund L . Detection of human and chick nuclear antigens in nuclei of chick erythrocytes during reactivation in heterokaryons with HeLa cells. Proc Natl Acad Sci U S A. 1971; 68(12):3228-32. PMC: 389627. DOI: 10.1073/pnas.68.12.3228. View

11.

Hynes R . Alteration of cell-surface proteins by viral transformation and by proteolysis. Proc Natl Acad Sci U S A. 1973; 70(11):3170-4. PMC: 427194. DOI: 10.1073/pnas.70.11.3170. View

12.

Wiblin C, Macpherson I . Reversion in hybrids between SV40-transformed hamster and mouse cells. Int J Cancer. 1973; 12(1):148-61. DOI: 10.1002/ijc.2910120116. View

13.

Vaheri A, Ruoslahti E . Disappearance of a major cell-type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by Rous sarcoma virus. Int J Cancer. 1974; 13(5):579-86. DOI: 10.1002/ijc.2910130502. View

14.

Wiener F, Klein G, Harris H . The analysis of malignancy by cell fusion. V. Further evidence of the ability of normal diploid cells to suppress malignancy. J Cell Sci. 1974; 15(1):177-83. DOI: 10.1242/jcs.15.1.177. View

15.

Wiener F, Klein G, Harris H . The analysis of malignancy by cell fusion. VI. Hybrids between different tumour cells. J Cell Sci. 1974; 16(1):189-98. DOI: 10.1242/jcs.16.1.189. View

16.

Hynes R, Bye J . Density and cell cycle dependence of cell surface proteins in hamster fibroblasts. Cell. 1974; 3(2):113-20. DOI: 10.1016/0092-8674(74)90114-7. View

17.

Veomett G, Prescott D, Shay J, PORTER K . Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B. Proc Natl Acad Sci U S A. 1974; 71(5):1999-2002. PMC: 388372. DOI: 10.1073/pnas.71.5.1999. View

18.

Klein G, Bregula U, Wiener F, Harris H . The analysis of malignancy by cell fusion. I. Hybrids between tumour cells and L cell derivatives. J Cell Sci. 1971; 8(3):659-72. DOI: 10.1242/jcs.8.3.659. View

19.

Weiss M, EPHRUSSI B . Studies of Interspecific (Rat x Mouse) Somatic Hybrids. I. Isolation, Growth and Evolution of the Karyotype. Genetics. 1966; 54(5):1095-109. PMC: 1211278. DOI: 10.1093/genetics/54.5.1095. View