New Phage-SPO1-induced Polypeptides Associated with Bacillus Subtilis RNA Polymerase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 Jul 1

PMID 4212197

Citations 19

Authors

T D Fox

J Pero

Affiliations

Soon will be listed here.

Abstract

RNA polymerase was precipitated from extracts of Bacillus subtilis infected with phage SP01 by antiserum prepared against core RNA polymerase. As shown by sodium dodecyl sulfate gel electrophoresis, the precipitates contained at least five new polypeptides not present in uninfected bacteria, in addition to the known subunits of RNA polymerase. The molecular weights of these polypeptides are (1) 85,000; (II) 40,000; (III) 28,000; (IV) 25,000; and (V) 23,000. Four of the polypeptides (I, III, IV, and V) co-purified with RNA polymerase through gel filtration and phosphocellulose chromatography. A pulse-chase experiment indicated that all five polypeptides are synthesized de novo after infection. The synthesis of polypeptides II, III, and IV commences almost immediately after infection, while polypeptides I and V first appear several minutes later. A sus mutant blocked early in transcription, susF21 [Fujita, et al. (1971) J. Mol. Biol. 57, 301-317] failed to induce polypeptides I, IV, and V, while two other mutants, susF4 and susF14, blocked late in transcription both failed only to induce polypeptide V.

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References

Burgess R, Travers A, Dunn J, Bautz E . Factor stimulating transcription by RNA polymerase. Nature. 1969; 221(5175):43-6. DOI: 10.1038/221043a0. View

Geiduschek E, Sklar J . Continual requirement for a host RNA polymerase component in a bacteriophage development. Nature. 1969; 221(5183):833-6. DOI: 10.1038/221833a0. View

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

Losick R, Sonenshein A . Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969; 224(5214):35-7. DOI: 10.1038/224035a0. View

Sonenshein A, Roscoe D . The course of phage phi-e infection in sporulating cells of Bacillus subtilis strain 3610. Virology. 1969; 39(2):265-75. DOI: 10.1016/0042-6822(69)90047-6. View

Gage L, Geiduschek E . RNA synthesis during bacteriophage SPO1 development: six classes of SPO1 RNA. J Mol Biol. 1971; 57(2):279-300. DOI: 10.1016/0022-2836(71)90346-9. View

Fujita D, Ohlsson-Wilhelm B, Geiduschek E . Transcription during bacteriophage SPO1 development: mutations affecting the program of viral transcription. J Mol Biol. 1971; 57(2):301-17. DOI: 10.1016/0022-2836(71)90348-2. View

Stevens A . New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1972; 69(3):603-7. PMC: 426516. DOI: 10.1073/pnas.69.3.603. View

Okubo S, Yanagida T, Fujita D . The genetics of bacteriophage SPO1. Biken J. 1972; 15(2):81-97. View

10.

Greenleaf A, Linn T, Losick R . Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis. Proc Natl Acad Sci U S A. 1973; 70(2):490-4. PMC: 433289. DOI: 10.1073/pnas.70.2.490. View

11.

Linn T, Greenleaf A, Shorenstein R, Losick R . Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation. Proc Natl Acad Sci U S A. 1973; 70(6):1865-9. PMC: 433614. DOI: 10.1073/pnas.70.6.1865. View

12.

Horvitz H . Polypeptide bound to the host RNA polymerase is specified by T4 control gene 33. Nat New Biol. 1973; 244(135):137-40. DOI: 10.1038/newbio244137a0. View

13.

CRAWFORD L, GESTELAND R . Synthesis of polyoma proteins in vitro. J Mol Biol. 1973; 74(4):627-34. DOI: 10.1016/0022-2836(73)90053-3. View

14.

Duffy J, Geiduschek E . Transcription specificity of an RNA polymerase fraction from bacteriophage SP01-infected B. subtilis. FEBS Lett. 1973; 34(2):172-4. DOI: 10.1016/0014-5793(73)80786-0. View

15.

Stevens A . Deoxyribonucleic acid dependent ribonucleic acid polymerases from two T4 phage-infected systems. Biochemistry. 1974; 13(3):493-503. DOI: 10.1021/bi00700a015. View

16.

Spiegelman G, WHITELEY H . Purification of ribonucleic acid polymerase from SP82-infected Bacillus subtilis. J Biol Chem. 1974; 249(5):1476-82. View

17.

Spiegelman G, WHITELEY H . In vivo and in vitro transcription by ribonucleic acid polymerase from SP82-infected Bacillus subtilis. J Biol Chem. 1974; 249(5):1483-9. View

18.

Holland M, WHITELEY H . RNA polymerase from Bacillus amyloliquefaciens infected with phi29 bacteriophage. Proc Natl Acad Sci U S A. 1973; 70(8):2234-7. PMC: 433708. DOI: 10.1073/pnas.70.8.2234. View

19.

Okubo S, Strauss B, STODOLSKY M . THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID. Virology. 1964; 24:552-62. DOI: 10.1016/0042-6822(64)90207-7. View