Effect of Temperature on the Distribution of Membrane Particles in Streptococcus Faecalis As Seen by the Freeze-fracture Technique

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 May 1

PMID 4208140

Citations 9

Authors

H C Tsien

M L Higgins

Affiliations

Soon will be listed here.

Abstract

When cells of Streptococcus faecalis ATCC 9790 were incubated at temperatures above 10 C before being frozen for freeze-fracture, a random distribution of particles was observed on the outer fracture face of the freeze-cleaved cell membrane. However, when cells were incubated below 10 C before freezing, particleless patches were seen on this membrane surface. The size of the patches produced on chilling could be increased by centrifugation or by storing the chilled cells overnight at about 3 C. Patch formation appeared readily reversible, since the medium and large patches that formed on chilling could not be observed in cells warmed for 10 s at 25 C. However, during the transition from the patch to patchless state, smaller patches not seen in the chilled cells were observed. This suggested that the smaller patches might have been intermediate forms produced by the fragmentation of larger patches on warming.

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Alterations in envelope structure of heptose-deficient mutants of Escherichia coli as revealed by freeze-etching.

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