Time Course of Purine Nucleoside Phosphorylase Occurrence in Sporulation of Bacillus Cereus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Jul 1

PMID 4204910

Citations 2

Authors

H L Englebrecht

Affiliations

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Abstract

Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only 3.3%. Conservation of the purine nucleoside phosphorylase during sporulation is apparent as shown by the gradual increase in heat resistance.

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References

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Gilpin R, SADOFF H . Physical and catalytic properties of the purine nucleoside phosphorylases from cells and spores of Bacillus cereus T. J Biol Chem. 1971; 246(5):1475-80. View