Simplified Method for Producing Pyocins from Pseudomonas Aeruginosa

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Jul 1

PMID 4199334

Citations 6

Authors

L F Jones

B V Pinto

E T Thomas

J J Farmer 3rd

Affiliations

Soon will be listed here.

Abstract

Good yields of pyocin are obtained when Pseudomonas aeruginosa is grown in Trypticase soy broth (without glucose) (BBL) plus 1% potassium nitrate. As a result, pyocin production for routine epidemiological typing can be done in screw-cap tubes, without mechanical agitation or mitomycin C induction.

Citing Articles

Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa.

Jacoby G Antimicrob Agents Chemother. 1974; 6(3):239-52.

PMID: 15830469 PMC: 444633. DOI: 10.1128/AAC.6.3.239.

Microbial opportunism in clinical medicine.

Poindexter H, Washington T J Natl Med Assoc. 1974; 66(4):284-91.

PMID: 4367817 PMC: 2609206.

Pyocin typing of Pseudomonas aeruginosa: a simplified method.

Jones L, Zakanycz J, Thomas E, Farmer 3rd J Appl Microbiol. 1974; 27(2):400-6.

PMID: 4207282 PMC: 380042. DOI: 10.1128/am.27.2.400-406.1974.

Pyocin sensitivity of Pseudomonas species.

Jones L, Thomas E, Stinnett J, Gilardi G, Farmer 3rd J Appl Microbiol. 1974; 27(1):288-9.

PMID: 4203785 PMC: 380013. DOI: 10.1128/am.27.1.288-289.1974.

Epidemiology of Pseudomonas aeruginosa in a general hospital: a four-year study.

Thomas E, Jones L, Simao E, SOLE-VERNIN C, Farmer 3rd J J Clin Microbiol. 1975; 2(5):397-402.

PMID: 811684 PMC: 274198. DOI: 10.1128/jcm.2.5.397-402.1975.

References

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GILLIES R, Govan J . Typing of Pseudomonas pyocyanea by pyocine production. J Pathol Bacteriol. 1966; 91(2):339-45. DOI: 10.1002/path.1700910207. View

STANIER R, Palleroni N, DOUDOROFF M . The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966; 43(2):159-271. DOI: 10.1099/00221287-43-2-159. View

Zabransky R, Day F . Pyocine typing of clinical strains of Pseudomonas aeruginosa. Appl Microbiol. 1969; 17(2):293-6. PMC: 377668. DOI: 10.1128/am.17.2.293-296.1969. View

Edmonds P, SUSKIND R, MacMILLAN B, Holder I . Epidemiology of Pseudomonas aeruginosa in a burns hospital: surveillance by a combined typing system. Appl Microbiol. 1972; 24(2):219-25. PMC: 380585. DOI: 10.1128/am.24.2.219-225.1972. View