A Possible Ribosomal-directed Regulatory System in Euglena Gracilis. Chlorophyll Synthesis

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Dec 1

PMID 4198358

Citations 2

Authors

M Perl

Affiliations

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Abstract

Cycloheximide at concentrations of 0.1-100mum stimulated chlorophyll synthesis when dark-grown cells of Euglena were illuminated. Chloramphenicol (1-4mm) inhibited chlorophyll synthesis. The effect of cycloheximide on the incorporation of [(14)C]leucine into material insoluble in trichloroacetic acid, and its failure to affect the incorporation of [(32)P]orthophosphate into such material in short incubations, are interpreted as evidence that cycloheximide specifically inhibits protein synthesis by 80S ribosomes. Since the inhibitory effect of chloramphenicol on chlorophyll synthesis is counteracted by the presence of cycloheximide, it is suggested that chlorophyll synthesis is subject to control by a cytoplasmic repressor synthesized on 80S ribosomes, and to a de-repressor synthesized on 70S ribosomes.

Citing Articles

Rubulose diphosphate carboxylase synthesis in Chlamydomonas reinhardii: Inhibition by chloramphenicol and stimulation by cycloheximide.

Givan A Planta. 2014; 120(2):181-8.

PMID: 24442657 DOI: 10.1007/BF00384928.

A possible ribosomal-directed regulatory system in Euglena gracilis. Carbon dioxide fixation.

Wolfovitch R, Perl M Biochem J. 1972; 130(3):819-23.

PMID: 4198359 PMC: 1174521. DOI: 10.1042/bj1300819.

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