A General Method for the Specific Staining of Intracellular Antigens with Ferritin-antibody Conjugates

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1970 Jan 1

PMID 4189988

Citations 23

Authors

J D McLean

S J Singer

Affiliations

Soon will be listed here.

Abstract

A general method is described whereby an intracellular macromolecule can be specifically stained with its antibody conjugated to ferritin. In this method cells or cell organelles are fixed, embedded in bovine serum albumin cross-linked with glutaraldehyde or formaldehyde, and then sectioned. This procedure preserves antigenic determinants as well as the cellular ultrastructure. The application of the ferritin-antibody conjugate to the section produces specific staining of the exposed antigen.

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