Early Stages in DNA Binding and Uptake During Genetic Transformation of Pneumococci

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 Apr 1

PMID 4151520

Citations 27

Authors

H Seto

A Tomasz

Affiliations

Soon will be listed here.

Abstract

Ethylenediaminetetraacetate and other divalent-cation-complexing agents greatly stimulate the cellular binding of DNA molecules to competent pneumococci, while the appearance of genetic transformants and nuclease-resistant DNA binding are completely inhibited. Based on this finding, we developed an experimental system in which three early and consecutive stages of genetic transformation can be experimentally separated: (i) attachment of DNA molecules to cell surface sites that are only demonstrable in the competent state; (ii) a divalent-cation-dependent nucleolytic splitting and release of the adsorbed molecules to the medium; and (iii) emergence of potential transformants accompanied by an energy-requiring and divalent-cation-dependent process in which the cell-associated DNA molecules become inaccessible to shearing forces, nucleases, anti-DNA serum, and polycations.

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