In Vitro Bactericidal and Associated Metabolic Activities of Mouse Spleen Cells

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1972 Jan 1

PMID 4144202

Citations 2

Authors

R R Strauss

B B Paul

A A Jacobs

A J Sbarra

Affiliations

Soon will be listed here.

Abstract

Spleen cell suspensions from AKR and CD-1 mice are able to kill Escherichia coli in vitro. The optimal ratio of splenocytes to bacteria for this activity is 1: 1. Incubation of these cells with inert polystyrene latex spherules (0.81 mum diameter) results in a fourfold increase in glucose-1-(14)C oxidation. Under these conditions, there is also a 2.5-fold increase in both reduced nicotinamide adenine dinucleotide phosphate oxidase activity and formate oxidation. Spleen cell fractions have been shown to have significant peroxidase activity. This has been quantitated by the guaiacol oxidation method. The 20,000 x g pellet fraction of spleen cell homogenate can kill E. coli when H(2)O(2) and chloride ions are added and the reaction is carried out at pH 5.5 and 37 C.

Citing Articles

Mouse splenic peroxidase and its role in bactericidal activity.

Strauss R, Paul B, Jacobs A, Sbarra A Infect Immun. 1972; 5(1):120-6.

PMID: 4632463 PMC: 422331. DOI: 10.1128/iai.5.1.120-126.1972.

Relationship between antigenic stimulation and increased splenic peroxidase levels during the immune response.

Strauss R, Friedman H, Mills L, Zayon G Infect Immun. 1977; 15(1):197-203.

PMID: 319058 PMC: 421349. DOI: 10.1128/iai.15.1.197-203.1977.

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