The Isocitrate Dehydrogenases of Acinetobacter Lwoffi. Separation and Properties of Two Nicotinamide-adenine Dinucleotide Phosphate-linked Isoenzymes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Nov 1

PMID 4144072

Citations 5

Authors

C H Self

P D Weitzman

Affiliations

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Abstract

Two isoenzymes of NADP-linked isocitrate dehydrogenase have been identified in Acinetobacter lwoffi and have been termed isoenzyme-I and isoenzyme-II. The isoenzymes may be separated by ion-exchange chromatography on DEAE-cellulose, by gel filtration on Sephadex G-200, or by zonal ultracentrifugation in a sucrose gradient. Low concentrations of glyoxylate or pyruvate effect considerable stimulation of the activity of isoenzyme-II. The isoenzymes also differ in pH-dependence of activity, kinetic parameters, stability to heat or urea and molecular size. Whereas isoenzyme-I resembles the NADP-linked isocitrate dehydrogenases from other organisms in having a molecular weight under 100000, isoenzyme-II is a much larger enzyme (molecular weight around 300000) resembling the NAD-linked isocitrate dehydrogenases of higher organisms.

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References

Reeves H, Brehmeyer B, AJL S . Multiple forms of bacterial NADP-specific isocitrate dehydrogenase. Science. 1968; 162(3851):359-60. DOI: 10.1126/science.162.3851.359. View

Rowe J, Reeves H . Electrophoretic heterogeneity of bacterial nicotinamide adenine dinucleotide phosphate-specific isocitrate dehydrogenases. J Bacteriol. 1971; 108(2):824-7. PMC: 247147. DOI: 10.1128/jb.108.2.824-827.1971. View

Colman R . Effect of modification of a methionyl residue on the kinetic and molecular properties of isocitrate dehydrogenase. J Biol Chem. 1968; 243(10):2454-64. View

HENDERSON N . Intracellular location and genetic control of isozymes of NADP-dependent isocitrate dehydrogenase and malate dehydrogenase. Ann N Y Acad Sci. 1968; 151(1):429-40. DOI: 10.1111/j.1749-6632.1968.tb11906.x. View

Giorgio Jr N, Yip A, FLEMING J, Plaut G . Diphosphopyridine nucleotide-linked isocitrate dehydrogenase from bovine heart. Polymeric forms and subunits. J Biol Chem. 1970; 245(20):5469-77. View

Parker M, Weitzman P . Regulation of NADP-linked isocitrate dehydrogenase activity in Acinetobacter. FEBS Lett. 1970; 7(4):324-326. DOI: 10.1016/0014-5793(70)80195-8. View

Baumann P, DOUDOROFF M, STANIER R . A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J Bacteriol. 1968; 95(5):1520-41. PMC: 252171. DOI: 10.1128/jb.95.5.1520-1541.1968. View

Barrera C, Jurtshuk P . Characterization of the highly active isocitrate (NADP+) dehydrogenase of Azotobacter vinelandii. Biochim Biophys Acta. 1970; 220(3):416-29. DOI: 10.1016/0005-2744(70)90273-1. View

VARGUES H, BRISOU J . [Action of sodium chloride on the isocitric dehydrogenase activity in 3 strains of halophile bacteria]. C R Seances Soc Biol Fil. 1966; 160(11):2195-9. View

10.

Self C, Weitzman P . Separation of isoenzymes by zonal centrifugation. Nature. 1970; 225(5233):644-5. DOI: 10.1038/225644a0. View

11.

SANWAL B, STACHOW C . ALLOSTERIC ACTIVATION OF NICOTINAMIDE-ADENINE DINUCLEOTIDE SPECIFIC ISOCITRATE DEHYDROGENASE OF NEUROSPORA. Biochim Biophys Acta. 1965; 96:28-44. DOI: 10.1016/0005-2787(65)90606-4. View

12.

CHUNG A, Franzen J . Oxidized triphosphopyridine nucleotide specific isocitrate dehydrogenase from Azotobacter vinelandii. Isolation and characterization. Biochemistry. 1969; 8(8):3175-84. DOI: 10.1021/bi00836a007. View

13.

Howard R, Becker R . Isolation and some properties of the triphosphopyridine nucleotide isocitrate dehydrogenase from Bacillus stearothermophilus. J Biol Chem. 1970; 245(12):3186-94. View

14.

Cox G, Davies D . The effects of pH and citrate on the activity of nicotinamideA-denine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria. Biochem J. 1969; 113(5):813-20. PMC: 1184771. DOI: 10.1042/bj1130813. View

15.

Siebert G, Dubuc J, Warner R, Plaut G . The preparation of isocitric dehydrogenase from mammalian heart. J Biol Chem. 1957; 226(2):965-75. View

16.

Kornberg H, PHIZACKERLEY P, SADLER J . The metabolism of C2 compounds in micro-organisms. 5. Biosynthesis of cell materials from acetate in Escherichia coli. Biochem J. 1960; 77:438-45. PMC: 1205054. DOI: 10.1042/bj0770438. View

17.

Barnes L, Kuehn G, Atkinson D . Yeast diphosphopyridine nucleotide specific isocitrate dehydrogenase. Purification and some properties. Biochemistry. 1971; 10(21):3939-44. DOI: 10.1021/bi00797a022. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Dixon M, Moyle J . Purification of the isocitric enzyme (triphosphopyridine nucleotide-linked isocitric dehydrogenase-oxalosuccinic carboxylase). Biochem J. 1956; 63(4):548-52. PMC: 1216216. DOI: 10.1042/bj0630548. View

20.

MAGAR M, Robbins J . The subunits of porcine heart TPN-linked isocitrate dehydrogenase. Biochim Biophys Acta. 1969; 191(1):173-6. DOI: 10.1016/0005-2744(69)90330-1. View