Crystal Formation by a Ribonucleic Acid Polymerase Mutant of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Oct 1

PMID 4126822

Citations 5

Authors

L Y Santo

R H Doi

Affiliations

Soon will be listed here.

Abstract

A crystalline inclusion has been observed in a ribonucleic acid polymerase mutant of Bacillus subtilis which is conditionally temperature sensitive only during sporulation. The crystal is formed at the permissive temperature in 1 to 2% of the sporulating (stage III-IV) cells; about 85% of the cells sporulate normally, while the cells with crystals do not sporulate. The wild type does not form crystals at either the permissive (30 C) or the nonpermissive (47 C) temperatures. The crystal may result from altered transcription during sporulation at 30 C.

Citing Articles

Unique regulation of crystal protein production in Bacillus thuringiensis subsp. yunnanensis is mediated by the cry protein-encoding 103-megadalton plasmid.

Srinivas G, Vennison S, Sudha S, Balasubramanian P, Sekar V Appl Environ Microbiol. 1997; 63(7):2792-7.

PMID: 9212426 PMC: 168575. DOI: 10.1128/aem.63.7.2792-2797.1997.

Ultrastructural analysis during germination and outgrowth of Bacillus subtilis spores.

Santo L, Doi R J Bacteriol. 1974; 120(1):475-81.

PMID: 4213660 PMC: 245785. DOI: 10.1128/jb.120.1.475-481.1974.

Crystal protein formed by Bacillus subtilis cells.

Rubikas J, Androsiuniene D, Chestukhina G, Smirnova T, Kapitonova O, Stepanov V J Bacteriol. 1987; 169(11):5258-62.

PMID: 3117772 PMC: 213934. DOI: 10.1128/jb.169.11.5258-5262.1987.

Alteration of tyrosine isoaccepting transfer ribonucleic acid species in wild-type and asporogenous strains of Bacillus subtilis.

McMillian R, ARCENEAUX J J Bacteriol. 1975; 122(2):526-31.

PMID: 805123 PMC: 246087. DOI: 10.1128/jb.122.2.526-531.1975.

Structure and morphogenesis of the bacterial spore coat.

Aronson A, Fitz-James P Bacteriol Rev. 1976; 40(2):360-402.

PMID: 786255 PMC: 413961. DOI: 10.1128/br.40.2.360-402.1976.

References

Holmes K, MONRO R . Studies on the structure of parasporal inclusions from Bacillus thuringiensis. J Mol Biol. 1965; 14(2):572-81. DOI: 10.1016/s0022-2836(65)80205-4. View

Somerville H, Delafield F, RITTENBERG S . Biochemical homology between crystal and spore protein of Bacillus thuringiensis. J Bacteriol. 1968; 96(3):721-6. PMC: 252364. DOI: 10.1128/jb.96.3.721-726.1968. View

Doi R, Brown L, Rodgers G, Hsu Y . Bacillus subtilis mutant altered in spore morphology and in RNA polymerase activity. Proc Natl Acad Sci U S A. 1970; 66(2):404-10. PMC: 283059. DOI: 10.1073/pnas.66.2.404. View

Korch C, Doi R . Electron microscopy of the altered spore morphology of a ribonucleic acid polymerase mutant of Bacillus subtilis. J Bacteriol. 1971; 105(3):1110-8. PMC: 248542. DOI: 10.1128/jb.105.3.1110-1118.1971. View

Leighton T . An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1973; 70(4):1179-83. PMC: 433452. DOI: 10.1073/pnas.70.4.1179. View