Genetic Control of Quantitative Variation in Carbonic Anhydrase Isozymes of Mammals. I. The Concentration Within Single Erythrocytes of the Mouse

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1977 Oct 1

PMID 412493

Citations 2

Authors

S S Hsu

R E TASHIAN

Affiliations

Soon will be listed here.

Abstract

The levels of the carbonic anhydrase isozymes (CA and CA II) in single erythrocytes of DBA/2J mice were assayed by measuring the specific immunofluorescence of CA I and CA II with a microspectrofluorometric technique. Measurements of 100 randomly selected cells showed a range (in relative fluorescence units) of 21-52 (mean 31.3 +/- 7.5) for CA I and 30-80 (mean 45.6 +/- 10.7) for CA II. The CA II/CA I ratio of the means obtained by the single-cell fluorescence assay was similar to the ratios obtained for the two isozymes from hemolysates of DBA/2J mice using an immunodiffusion assay. The influence of cell age on the variation in carbonic anhydrase levels was determined by separating red cells into several fractions by a gravity sedimentation procedure. The younger cells showed higher levels of CA I and CA II than the older cells; however, the extensive overlap in the variability between the cells from the different fractions indicated that although cell age was contributing to the overall heterogeneity, its influence was not pronounced.

Citing Articles

Carbonic anhydrase: an ultrastructural study in rat cerebellum.

Langley O, Ghandour M, Vincendon G, Gombos G Histochem J. 1980; 12(4):473-83.

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Immunohistochemical localization of carbonic anhydrase I and II in eccrine sweat glands from control subjects and patients with cystic fibrosis.

Briggman J, TASHIAN R, SPICER S Am J Pathol. 1983; 112(3):250-7.

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