Alpha 2.0
Login Register
» Articles » PMID: 41243

A New GlnA-linked Regulatory Gene for Glutamine Synthetase in Escherichia Coli

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 1979 Sep 1
PMID 41243
Citations 82
Authors
G Pahel
B Tyler
Affiliations
Soon will be listed here.
Abstract

Mutations in the glnA region of the Escherichia coli chromosome due to Mu prophage insertion result in two phenotypic classes. One class is Gln- and does not synthesize glutamine synthetase[L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] under any growth condition. The other class produces a low level of glutamine synthetase under all growth conditions and is uncoupled from the regulatory effects of mutations in the glnF and glnD genes. Complementation analysis demonstrates that these two classes of insertions are in different cistrons. From these data we suggest that a regulatory gene, glnG, tightly linked to glnA, mediates both activation and repression of glutamine synthetase synthesis. An analysis of the evidence accumulated to date makes it unlikely that glnG is the only gene in the glnA region involved in the complex system of nitrogen regulation.

Citing Articles

Metabolic engineering of Escherichia coli for efficient production of L-alanyl-L-glutamine.

Zhu J, Yang W, Wang B, Liu Q, Zhong X, Gao Q Microb Cell Fact. 2020; 19(1):129.

PMID: 32527330 PMC: 7291740. DOI: 10.1186/s12934-020-01369-2.

Response regulator VemR regulates the transcription of flagellar rod gene flgG by interacting with σ factor RpoN2 in Xanthomonas citri ssp. citri.

Wu W, Zhao Z, Luo X, Fan X, Zhuo T, Hu X Mol Plant Pathol. 2018; 20(3):372-381.

PMID: 30353625 PMC: 6637908. DOI: 10.1111/mpp.12762.

Co-regulation of Iron Metabolism and Virulence Associated Functions by Iron and XibR, a Novel Iron Binding Transcription Factor, in the Plant Pathogen Xanthomonas.

Pandey S, Patnana P, Lomada S, Tomar A, Chatterjee S PLoS Pathog. 2016; 12(11):e1006019.

PMID: 27902780 PMC: 5130282. DOI: 10.1371/journal.ppat.1006019.

Proteomic View of Interactions of Shiga Toxin-Producing Escherichia coli with the Intestinal Environment in Gnotobiotic Piglets.

Pieper R, Zhang Q, Clark D, Parmar P, Alami H, Suh M PLoS One. 2013; 8(6):e66462.

PMID: 23840478 PMC: 3686733. DOI: 10.1371/journal.pone.0066462.

Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation.

Salinero K, Keller K, Feil W, Feil H, Trong S, Di Bartolo G BMC Genomics. 2009; 10:351.

PMID: 19650930 PMC: 2907700. DOI: 10.1186/1471-2164-10-351.

References
1.
Taylor A . BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963; 50:1043-51. PMC: 221271. DOI: 10.1073/pnas.50.6.1043. View
2.
Weglenski P, Tyler B . Regulation of glnA messinger ribonucleic acid synthesis in Klebsiella aerogenes. J Bacteriol. 1977; 129(2):880-7. PMC: 235025. DOI: 10.1128/jb.129.2.880-887.1977. View
3.
Garcia E, Bancroft S, Rhee S, Kustu S . The product of a newly identified gene, gInF, is required for synthesis of glutamine synthetase in Salmonella. Proc Natl Acad Sci U S A. 1977; 74(4):1662-6. PMC: 430852. DOI: 10.1073/pnas.74.4.1662. View
4.
Bender R, MAGASANIK B . Regulatory mutations in the Klebsiella aerogenes structural gene for glutamine synthetase. J Bacteriol. 1977; 132(1):100-5. PMC: 221831. DOI: 10.1128/jb.132.1.100-105.1977. View
5.
Pahel G, Zelenetz A, Tyler B . gltB gene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli. J Bacteriol. 1978; 133(1):139-48. PMC: 221987. DOI: 10.1128/jb.133.1.139-148.1978. View