Studies on the Adsorption of Certain Medium Proteins to Mycoplasma Gallisepticum and Their Influence on Agglutination and Haemagglutination Reactions

Overview

Journal J Hyg (Lond)

Publisher Cambridge University Press

Specialty Public Health

Date 1972 Jun 1

PMID 4113479

Citations 18

Authors

J M Bradbury

F T Jordan

Affiliations

Soon will be listed here.

Abstract

Serum proteins from Mycoplasma gallisepticum culture medium could be detected on the organisms as a result of incubation at low pH. Only certain of the serum proteins, including IgG and IgM, were found, and the adsorption appears to depress the haemagglutinating activity of the organisms. There was no obvious effect on slide agglutination (SA) sensitivity but an incidental finding was that brief acid treatment enhanced the SA sensitivity of an antigen prepared from a young culture.

Citing Articles

Factors influencing the cell adhesion and invasion capacity of Mycoplasma gallisepticum.

Furnkranz U, Siebert-Gulle K, Rosengarten R, Szostak M Acta Vet Scand. 2013; 55:63.

PMID: 24011130 PMC: 3847126. DOI: 10.1186/1751-0147-55-63.

Membrane-associated nuclease activities in mycoplasmas.

Minion F, Billings D, Tigges E J Bacteriol. 1993; 175(24):7842-7.

PMID: 8253673 PMC: 206960. DOI: 10.1128/jb.175.24.7842-7847.1993.

Studies on the effect of growth medium composition on the antigenicity of Mycoplasma bovis.

Thorns C, Boughton E J Hyg (Lond). 1980; 84(1):29-36.

PMID: 6766155 PMC: 2133824. DOI: 10.1017/s0022172400026486.

Medium components adsorbed to mycoplasmal cells.

Sugiyama T Yale J Biol Med. 1983; 56(5-6):691-3.

PMID: 6433581 PMC: 2590558.

Isolation, ultrastructure and antigenicity of Mycoplasma gallisepticum membranes.

Levisohn S, Razin S J Hyg (Lond). 1973; 71(4):725-37.

PMID: 4520511 PMC: 2130413. DOI: 10.1017/s0022172400022981.

References

Smith S, Dunlop W, STROUT R . Effect of culture medium on antigenic structure of mycoplasma. Avian Dis. 1966; 10(2):173-6. View

Tormo J, Chordi A, Rodriguez-Burgos A, Diaz R . Immunoelectrophoresis of normal pig serum. Vet Rec. 1967; 81(16):392-5. DOI: 10.1136/vr.81.16.392. View

King C . A general study on the adsorption of protein by tissue. Biochim Biophys Acta. 1968; 154(2):269-77. DOI: 10.1016/0005-2795(68)90040-8. View

Beernink K, STEWARD J . Proteins adhering to Escherichia coli after exposure to guinea-pig serum. Immunology. 1967; 12(2):207-18. PMC: 1409272. View

Jordan F, Kulasegaram P . Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. J Comp Pathol. 1968; 78(4):407-14. DOI: 10.1016/0021-9975(68)90038-8. View

Bradbury J, Jordan F . The absorption of -globulins to Mycoplasma gallisepticum and the possible role in non-specific serological reactions. Vet Rec. 1971; 89(11):318. DOI: 10.1136/vr.89.11.318. View

Bradbury J, Jordan F . The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests. J Hyg (Lond). 1971; 69(4):593-606. PMC: 2131049. DOI: 10.1017/s0022172400021872. View

Hamburger R, PIOUS D, Mills S . ANTIGENIC SPECIFICITIES ACQUIRED FROM THE GROWTH MEDIUM BY CELLS IN TISSUE CULTURE. Immunology. 1963; 6:439-49. PMC: 1423289. View

SCHEIDEGGER J . [A micro-method of immuno-electrophoresis]. Int Arch Allergy Appl Immunol. 1955; 7(2):103-10. View

10.

NEIMARK H, Pickett M . Products of glucose metabolism by pleuropneumonialike organisms. Ann N Y Acad Sci. 1960; 79:531-7. DOI: 10.1111/j.1749-6632.1960.tb42719.x. View

11.

Tourtellotte M, Jacobs R . Physiological and serologic comparisons of PPLO from various sources. Ann N Y Acad Sci. 1960; 79:521-30. DOI: 10.1111/j.1749-6632.1960.tb42718.x. View