Structure and Replication of Chromosomes in Competent Cells of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Mar 1

PMID 4110922

Citations 17

Authors

R J Erickson

J C Copeland

Affiliations

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Abstract

Competent cultures of Bacillus subtilis 168 were fractionated on gradients of Renografin-76 to obtain a population enriched for competent cells. The cells in this fraction contained two nuclear bodies. The competent cell fraction synthesized deoxyribonucleic acid and ribonucleic acid at reduced rates compared to the noncompetent cell fraction and appeared to divide synchronously upon incubation. The state of the chromosome in competent cells was determined by density transfer experiments and marker frequency analyses. The results are consistent with a competent cell possessing two, or a multiple of two, chromosomes, one complete and the other partially duplicated. During subsequent growth the partially completed chromosome replicates preferentially.

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References

Anagnostopoulos C, Spizizen J . REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961; 81(5):741-6. PMC: 279084. DOI: 10.1128/jb.81.5.741-746.1961. View

Dubnau D, Goldthwaite C, Smith I, MARMUR J . Genetic mapping in Bacillus subtilis. J Mol Biol. 1967; 27(1):163-85. DOI: 10.1016/0022-2836(67)90358-0. View

Sueoka N, Yoshikawa H . The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics. 1965; 52(4):747-57. PMC: 1210937. DOI: 10.1093/genetics/52.4.747. View

Cahn F, Fox M . Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968; 95(3):867-75. PMC: 252104. DOI: 10.1128/jb.95.3.867-875.1968. View

RAMALEY R, Burden L . Replacement sporulation of Bacillus subtilis 168 in a chemically defined medium. J Bacteriol. 1970; 101(1):1-8. PMC: 250444. DOI: 10.1128/jb.101.1.1-8.1970. View

Yoshikawa H, Sueoka N . Sequential replication of the Bacillus subtilis chromosome. II. Isotopic transfer experiments. Proc Natl Acad Sci U S A. 1963; 49:806-13. PMC: 300011. DOI: 10.1073/pnas.49.6.806. View

Hadden C, Nester E . Purification of competent cells in the Bacillus subtilis transformation system. J Bacteriol. 1968; 95(3):876-85. PMC: 252105. DOI: 10.1128/jb.95.3.876-885.1968. View

Kogoma T, Yanagita T . Nonselective incorporation into sporangium of either "older" or "younger" chromosome of the vegetative cell during sporulation in Bacillus cereus. J Bacteriol. 1967; 94(5):1715-21. PMC: 276882. DOI: 10.1128/jb.94.5.1715-1721.1967. View

Javor G, Tomasz A . An autoradiographic study of genetic transformation. Proc Natl Acad Sci U S A. 1968; 60(4):1216-22. PMC: 224906. DOI: 10.1073/pnas.60.4.1216. View

10.

Bott K, Wilson G . Development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1967; 94(3):562-70. PMC: 251923. DOI: 10.1128/jb.94.3.562-570.1967. View

11.

Bott K, Wilson G . Metabolic and nutritional factors influencing the development of competence for transfection of Bacillus subtilis. Bacteriol Rev. 1968; 32(4 Pt 1):370-8. PMC: 408308. View

12.

Singh R, Pitale M . Competence and deoxyribonucleic acid uptake in Bacillus subtilis. J Bacteriol. 1968; 95(3):864-6. PMC: 252103. DOI: 10.1128/jb.95.3.864-866.1968. View

13.

Copeland J . Regulation of chromosome replication in Bacillus subtilis: effects of amino acid starvation in strain 168. J Bacteriol. 1969; 99(3):730-6. PMC: 250088. DOI: 10.1128/jb.99.3.730-736.1969. View

14.

McCarthy C, Nester E . Macromolecular synthesis in newly transformed cells of Bacillus subtilis. J Bacteriol. 1967; 94(1):131-40. PMC: 251881. DOI: 10.1128/jb.94.1.131-140.1967. View

15.

Copeland J, MARMUR J . Identification of conserved genetic functions in Bacillus by use of temperature-sensitive mutants. Bacteriol Rev. 1968; 32(4 Pt 1):302-12. PMC: 408302. View

16.

Laird C, Wang L, Bodmer W . Recombination and DNA replication in Bacillus subtilis transformation. Mutat Res. 1968; 6(2):205-9. DOI: 10.1016/0027-5107(68)90035-3. View

17.

Nester E, Stocker B . BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1963; 86:785-96. PMC: 278516. DOI: 10.1128/jb.86.4.785-796.1963. View

18.

Erickson R, Braun W . Apparent dependence of transformation on the stage of deoxyribonucleic acid replication of recipient cells. Bacteriol Rev. 1968; 32(4 Pt 1):291-6. PMC: 408300. View

19.

RYTER A, Aubert J . [Autoradiographic study of DNA synthesis during sporulation of Bacillus subtilis]. Ann Inst Pasteur (Paris). 1969; 117(5):601-11. View

20.

Bodmer W . Recombination and integration in Bacillus subtilis transformation: involvement of DNA synthesis. J Mol Biol. 1965; 14(2):534-57. DOI: 10.1016/s0022-2836(65)80203-0. View