Assignment of Three Human Genes to Chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and Evidence for Translocation Between Human and Mouse Chromosomes in Somatic Cell Hybrids (thymidine Kinase-lactate Dehydrogenase A-isocitrate Dehydrogenase-C-11,...

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Feb 1

PMID 4110482

Citations 44

Authors

C Boone

T R Chen

F H Ruddle

Affiliations

Soon will be listed here.

Abstract

Independently derived man-mouse somatic cell hybrids and their derivative subclones show a positive correlation between the expression of human lactate dehydrogenase A subunits and the occurrence of the human C-11 chromosome. Data are also presented that confirm the previously reported linkage of the thymidine kinase locus to the E-17 chromosome. A translocation of the E-17 chromosome provides presumptive evidence for the assignment of the thymidine kinase locus to the long arm segment of the E-17 chromosome. This translocation also provides evidence for translocation between man and mouse chromosomes in somatic cell hybrids. A presumptive association between the human phenotype for isocitrate dehydrogenase and the human F group is also described. Identification of specific human chromosomes was achieved by the application of several new cytological techniques: measurement of chromosome arm length, in situ annealing with mouse satellite complementary RNA, constitutive heterochromatin staining with Giemsa, and quinacrine mustard fluorochromatic staining.

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