The Osmotic Effects of Electron Microscope Fixatives

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1971 Jun 1

PMID 4103952

Citations 46

Authors

Q Bone

E J Denton

Affiliations

Soon will be listed here.

Abstract

The reflecting cells on the scales of sprat and herring contain ordered arrays of guanine crystals. The spacing of the crystals within these cells determines the wave bands of the light which they reflect, hence volume changes in the reflecting cells can be observed as color changes directly. This property of the scales is used to show that (a) fixation with osmium tetroxide solutions destroys osmotic activity; (b) fixation with aldehyde solutions does not destroy osmotic activity and does not cause volume changes if the aldehydes are made up in salt or sucrose solutions whose osmolarities, discounting the aldehyde, are about 60% of those to which the cells are in equilibrium in life, and (c) after aldehyde fixation the cells are osmotically active but come to a given volume in salt and sucrose solutions of concentrations only 60% of those which give their volume before fixation. Various possible mechanisms underlying the change of osmotic equilibrium caused by aldehyde fixation are discussed.

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References

MAUNSBACH A . The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. II. Effects of varying osmolality, ionic strength, buffer system and fixative concentration of glutaraldehyde solutions. J Ultrastruct Res. 1966; 15(3):283-309. DOI: 10.1016/s0022-5320(66)80110-7. View

ELBERS P . Ion permeability of the egg of Limnaea stagnalis L. on fixation for electron microscopy. Biochim Biophys Acta. 1966; 112(2):318-29. DOI: 10.1016/0926-6585(66)90330-x. View

Hopwood D . Some aspects of fixation with glutaraldehyde. A biochemical and histochemical comparison of the effects of formaldehyde and glutaraldehyde fixation on various enzymes and glycogen, with a note on penetration of glutaraldehyde into liver. J Anat. 1967; 101(Pt 1):83-92. PMC: 1270860. View

Lenard J, Singer S . Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy. J Cell Biol. 1968; 37(1):117-21. PMC: 2107400. DOI: 10.1083/jcb.37.1.117. View

HUDSON R . A ringer solution for Cottus (teleost) fast muscle fibres. Comp Biochem Physiol. 1968; 25(2):719-25. DOI: 10.1016/0010-406x(68)90382-4. View

Habeeb A, Hiramoto R . Reaction of proteins with glutaraldehyde. Arch Biochem Biophys. 1968; 126(1):16-26. DOI: 10.1016/0003-9861(68)90554-7. View

Fozzard H, Dominguez G . Effect of formaldehyde and glutaraldehyde on electrical properties of cardiac Purkinje fibers. J Gen Physiol. 1969; 53(5):530-40. PMC: 2202882. DOI: 10.1085/jgp.53.5.530. View

Palade G . A study of fixation for electron microscopy. J Exp Med. 1952; 95(3):285-98. PMC: 2212069. DOI: 10.1084/jem.95.3.285. View

Denton E, Walker M . The visual pigment of the conger eel. Proc R Soc Lond B Biol Sci. 1958; 148(931):257-69. DOI: 10.1098/rspb.1958.0017. View

10.

Schultz R, Karlsson U . FIXATION OF THE CENTRAL NERVOUS SYSTEM FOR ELECTRON MICROSCOPY BY ALDEHYDE PERFUSION. II. EFFECT OF OSMOLARITY, PH OF PERFUSATE, AND FIXATIVE CONCENTRATION. J Ultrastruct Res. 1965; 12:187-206. DOI: 10.1016/s0022-5320(65)80015-6. View

11.

TORACK R . THE EXTRACELLULAR SPACE OF RAT BRAIN FOLLOWING PERFUSION FIXATION WITH GLUTARALDEHYDE AND HYDROXYADIPALDEHYDE. Z Zellforsch Mikrosk Anat. 1965; 66(3):352-64. DOI: 10.1007/BF00334717. View

12.

Baker J . THE FINE STRUCTURE PRODUCED IN CELLS BY FIXATIVES. J R Microsc Soc. 1965; 84:115-31. DOI: 10.1111/j.1365-2818.1965.tb02114.x. View

13.

FRAENKEL-CONRAT H, Mecham D . The reaction of formaldehyde with proteins; demonstration of intermolecular cross-linking by means of osmotic pressure measurements. J Biol Chem. 1949; 177(1):477-86. View