Comparison of Animal Infectivity and Excystation As Measures of Giardia Muris Cyst Inactivation by Chlorine
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Microbiology
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In this study, in vitro excystation and mouse infectivity were compared as methods for quantitatively determining the viability of Giardia muris cysts before and after exposure to free residual chlorine. The mouse infectivity results show that very few cysts (1 to 15) constitute an infectious dose. The results of the inactivation studies indicate that in vitro excystation is an adequate indication of G. muris cyst infectivity for the host and can be used to determine the effects of disinfectants on cyst viability.
Priest J, Moss D, Visvesvara G, Jones C, Li A, Isaac-Renton J Clin Vaccine Immunol. 2010; 17(11):1695-707.
PMID: 20876825 PMC: 2976096. DOI: 10.1128/CVI.00160-10.
Biocidal efficacy of a flocculating emergency water purification tablet.
Powers E, Hernandez C, Boutros S, Harper B Appl Environ Microbiol. 1994; 60(7):2316-23.
PMID: 16349318 PMC: 201649. DOI: 10.1128/aem.60.7.2316-2323.1994.
Efficacy of two peroxygen-based disinfectants for inactivation of Cryptosporidium parvum oocysts.
Quilez J, Sanchez-Acedo C, Avendano C, Del Cacho E, Lopez-Bernad F Appl Environ Microbiol. 2005; 71(5):2479-83.
PMID: 15870337 PMC: 1087572. DOI: 10.1128/AEM.71.5.2479-2483.2005.
Detection of viable Giardia cysts by amplification of heat shock-induced mRNA.
Abbaszadegan M, Huber M, Gerba C, Pepper I Appl Environ Microbiol. 1997; 63(1):324-8.
PMID: 8979360 PMC: 168324. DOI: 10.1128/aem.63.1.324-328.1997.
Wallis P, Erlandsen S, Olson M, Robertson W, van Keulen H Appl Environ Microbiol. 1996; 62(8):2789-97.
PMID: 8702271 PMC: 168064. DOI: 10.1128/aem.62.8.2789-2797.1996.