Preparation of 67Ga-labelled Human IgG and Its Fab Fragments Using Desferoxamine As Chelating Agent

Overview

Journal Eur J Nucl Med

Specialty Nuclear Medicine

Date 1985 Jan 1

PMID 4076230

Citations 2

Authors

S A Eccles

C Dean

G Coghlan

Affiliations

Soon will be listed here.

Abstract

Human IgG and its Fab fragments were chosen as a model system for studying the desferoxamine (DF) coupling reaction using glutaraldehyde or carbodiimide at various concentrations. The labelling of the IgG-DF conjugates with 67Ga proved to be highly efficient and reproducible. The labelling efficiency in relation to storage time of the conjugate was analysed over a 1-month period by Sephadex-G-50 chromatography. Gel-filtration analysis on Sephacryl S-300 showed that, following the coupling procedure, a high proportion (more than 80%) of the conjugate remained monomeric. Immunoelectrophoresis on agar plates demonstrated that the antibody immunoreactivity was preserved. The biodistribution in mice of the 67Ga-DF-IgG conjugates was comparable to that of the conventional radiopharmaceutical, 125I-HSA. Conditions were established for the coupling of DF to two rat IgG2b monoclonal antibodies M10/76 and 11/160, that are specific for the Hooded rat sarcomata MC 24 and HSN respectively; the immunoreactivity of these conjugates was tested by a cell-binding assay. The data indicate that monoclonal-antibody/DF conjugates prepared with the bifunctional reagent glutaraldehyde maintain their capacity for binding to their tumour-associated antigens.

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