Poly(dG)-poly(dC) Sequences, Under Torsional Stress, Induce an Altered DNA Conformation Upon Neighboring DNA Sequences

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 1985 Nov 1

PMID 4075394

Citations 30

Authors

T Kohwi-Shigematsu

Y Kohwi

Affiliations

Soon will be listed here.

Abstract

Supercoiled plasmid DNAs (at bacterial superhelical density) harboring the homopurine-homopyrimidine sequence, poly(dG)-poly(dC), were reacted with bromoacetaldehyde (BAA), a reagent that reacts with unpaired DNA bases. Not only did the poly(dG)-poly(dC) sequence react with BAA but, surprisingly, neighboring sequences located 3' to the contiguous G sequences also reacted. The altered conformation in the poly(dG)-poly(dC) sequence and in the neighboring sequence occurred in the same supercoiled plasmid DNA molecule. Furthermore, the occurrence of an "unpaired" conformation in the neighboring sequence is strictly due to a positional effect, since it is observed when the poly(dG)-poly(dC) segment is adjacent to a variety of neighboring sequences.

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