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Microneutralization Test for Respiratory Syncytial Virus Based on an Enzyme Immunoassay

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1985 Dec 1
PMID 4066916
Citations 39
Authors
L J Anderson
J C Hierholzer
P G Bingham
Y O Stone
Affiliations
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Abstract

Virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. After an appropriate incubation period, the cells were fixed, and an enzyme-linked immunosorbent assay was performed directly in the microtiter plates for detection of virus. Results could be read and recorded automatically, which is especially helpful when running large numbers of tests.

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References
1.
PURSELL A, Cole Jr J . Procedure for fluorescent-antibody staining of virus-infected cell cultures in plastic plates. J Clin Microbiol. 1976; 3(5):537-40. PMC: 274345. DOI: 10.1128/jcm.3.5.537-540.1976. View
2.
Wyatt R, James W, BOHL E, Theil K, Saif L, Kalica A . Human rotavirus type 2: cultivation in vitro. Science. 1980; 207(4427):189-91. DOI: 10.1126/science.6243190. View
3.
Gerna G, Cereda P, Revello M, Cattaneo E, Battaglia M, Gerna M . Antigenic and biological relationships between human coronavirus OC43 and neonatal calf diarrhoea coronavirus. J Gen Virol. 1981; 54(Pt 1):91-102. DOI: 10.1099/0022-1317-54-1-91. View
4.
Lemon S, Binn L, MARCHWICKI R . Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures. J Clin Microbiol. 1983; 17(5):834-9. PMC: 272750. DOI: 10.1128/jcm.17.5.834-839.1983. View
5.
CHANOCK R, Roizman B, Myers R . Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am J Hyg. 1957; 66(3):281-90. DOI: 10.1093/oxfordjournals.aje.a119901. View