Methods for Study of Mutations and Mutagenesis in Human Lymphocytes

Overview

Journal Mutat Res

Publisher Elsevier

Specialty Genetics

Date 1985 Dec 1

PMID 4058450

Citations 5

Authors

A A Morley

K J Trainor

J L Dempsey

R S Seshadri

Affiliations

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Abstract

Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing interleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.

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Shanahan E, Peterson D, Roxby D, Quintana J, Morely A, Woodward A Occup Environ Med. 1996; 53(7):439-44.

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Mutagenesis after cancer therapy.

Kelsey K, Caggana M, Mauch P, Coleman C, Clark J, Liber H Environ Health Perspect. 1993; 101 Suppl 3:177-84.

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Modulation of antifolate cytotoxicity by metabolites from dying cells in a lymphocyte clonal assay.

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Mutations in human lymphocytes commonly involve gene duplication and resemble those seen in cancer cels.

Turner D, Grist S, Janatipour M, Morley A Proc Natl Acad Sci U S A. 1988; 85(9):3189-92.

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Mutagenic activity of BKV and JCV in human and other mammalian cells.

Theile M, Grabowski G Arch Virol. 1990; 113(3-4):221-33.

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