Radioimmunoassay for Clonidine in Human Plasma and Urine Using a Solid Phase Second Antibody Separation

Overview

Journal Ther Drug Monit

Specialty Pharmacology

Date 1985 Jan 1

PMID 4049475

Citations 3

Authors

P R Farina

C A Homon

C T Chow

J J Keirns

P A Zavorskas

H J Esber

Affiliations

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Abstract

A reliable, sensitive, and specific radioimmunoassay (RIA) procedure for the quantitation of clonidine in plasma and other biological fluids was developed. The detection limit of the assay is 2 pg based on a 200 microliters sample. Nine commonly used drugs were found not to interfere with the RIA. The utility of the assay was demonstrated in a bioavailability study of clonidine conducted with 24 healthy subjects. Clonidine was readily quantitated in plasma over 4 half-lives. This assay is suitable for pharmacokinetic and bioavailability studies as well as therapeutic drug monitoring of patients.

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