Isolation of Capnocytophaga Species with a New Selective Medium

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1985 Sep 1

PMID 4044796

Citations 1

Authors

J L Rummens

J M Fossepre

M De Gruyter

H Van de Vyver

L Neyt

H W Van Landuyt

Affiliations

Soon will be listed here.

Abstract

A selective medium (CAP) composed of a GC agar base supplemented with 1% hemoglobin, 1% Polyvitex, and an antibiotic mixture of polymyxin B (15 U/ml), vancomycin (5 micrograms/ml), trimethoprim (2.5 micrograms/ml), and amphotericin B (2.5 micrograms/ml) was compared with another selective medium (TBBP) and two nonselective media--a blood agar and a chocolate agar--to isolate Capnocytophaga species from 725 clinical specimens. These included sputa (467 specimens), throat swabs (116 specimens), oral ulcerations (35 specimens), and periodontal pockets (107 specimens). The recovery rate of Capnocytophaga species was significantly higher with the CAP medium (96%) than with the selective TBBP medium (52.2%), the nonselective blood agar (6.2%), and the chocolate agar (4.6%). Growth of the normal flora was best inhibited on CAP medium. Colony size and yellow-brown pigment formation were maximally expressed on chocolate agar and CAP medium, but gliding motility was mostly absent. We conclude that the CAP medium is an excellent medium for the recovery of Capnocytophaga species from contaminated clinical specimens.

Citing Articles

In vitro susceptibility of Capnocytophaga species to 29 antimicrobial agents.

Rummens J, Gordts B, Van Landuyt H Antimicrob Agents Chemother. 1986; 30(5):739-42.

PMID: 3800350 PMC: 176524. DOI: 10.1128/AAC.30.5.739.

References

Rummens J, Verfaillie C, Louwagie A, Boelaert J, Van Landuyt H . Capnocytophaga infection: a risk in the immunocompromised host. Acta Clin Belg. 1984; 39(2):109-13. DOI: 10.1080/22953337.1984.11718990. View

Winn R, Chase W, Lauderdale P, McCleskey F . Septic arthritis involving Capnocytophaga ochracea. J Clin Microbiol. 1984; 19(4):538-40. PMC: 271112. DOI: 10.1128/jcm.19.4.538-540.1984. View

Kristiansen J, Bremmelgaard A, Busk H, Heltberg O, Frederiksen W, Justesen T . Rapid identification of Capnocytophaga isolated from septicemic patients. Eur J Clin Microbiol. 1984; 3(3):236-40. DOI: 10.1007/BF02014892. View

Newman M, Socransky S, Savitt E, Propas D, Crawford A . Studies of the microbiology of periodontosis. J Periodontol. 1976; 47(7):373-9. DOI: 10.1902/jop.1976.47.7.373. View

Appelbaum P, Ballard J, Eyster M . Septicemia due to Capnocytophaga (Bacteroides ocharaceus) in Hodgkin's disease. Ann Intern Med. 1979; 90(4):716-7. DOI: 10.7326/0003-4819-90-4-716. View

Holt S, Leadbetter E, Socransky S . Capnocytophaga: new genus of gram-negative gliding bacteria. II. Morphology and ultrastructure. Arch Microbiol. 1979; 122(1):17-27. DOI: 10.1007/BF00408041. View

Socransky S, Holt S, Leadbetter E, Tanner A, Savitt E, HAMMOND B . Capnocytophaga: new genus of gram-negative gliding bacteria. III. Physiological characterization. Arch Microbiol. 1979; 122(1):29-33. DOI: 10.1007/BF00408042. View

Williams B, HAMMOND B . Capnocytophaga: new genus of gram-negative gliding bacteria. IV. DNA base composition and sequence homology. Arch Microbiol. 1979; 122(1):35-9. DOI: 10.1007/BF00408043. View

Leadbetter E, Holt S, Socransky S . Capnocytophaga: new genus of gram-negative gliding bacteria. I. General characteristics, taxonomic considerations and significance. Arch Microbiol. 1979; 122(1):9-16. DOI: 10.1007/BF00408040. View

10.

Newman M, Sutter V, Pickett M, Blachman U, Greenwood J, Grinenko V . Detection, identification, and comparison of Capnocytophaga, Bacteroides ochraceus, and DF-1. J Clin Microbiol. 1979; 10(4):557-62. PMC: 273214. DOI: 10.1128/jcm.10.4.557-562.1979. View

11.

Forlenza S, Newman M, LIPSEY A, Siegel S, Blachman U . Capnocytophaga sepsis: a newly recognised clinical entity in granulocytopenic patients. Lancet. 1980; 1(8168 Pt 1):567-8. DOI: 10.1016/s0140-6736(80)91057-0. View

12.

Haulk A, Sugar A, Rushing J . Capnocytophaga septicemia. N Engl J Med. 1980; 302(16):922. DOI: 10.1056/NEJM198004173021618. View

13.

Gandola C, Butler T, Badger S, Cheng E, Beard S . Septicemia caused by Capnocytophaga in a granulocytopenic patient with glossitis. Arch Intern Med. 1980; 140(6):851-2. View

14.

Gilligan P, McCarthy L, Bissett B . Capnocytophaga ochracea septicemia. J Clin Microbiol. 1981; 13(4):643-5. PMC: 273851. DOI: 10.1128/jcm.13.4.643-645.1981. View

15.

Forlenza S, Newman M, Horikoshi A, Blachman U . Antimicrobial susceptibility of Capnocytophaga. Antimicrob Agents Chemother. 1981; 19(1):144-6. PMC: 181373. DOI: 10.1128/AAC.19.1.144. View

16.

Sutter V, Pyeatt D, Kwok Y . In vitro susceptibility of Capnocytophaga strains to 18 antimicrobial agents. Antimicrob Agents Chemother. 1981; 20(2):270-1. PMC: 181679. DOI: 10.1128/AAC.20.2.270. View

17.

De Rautlin De La Roy Y, Grignon B, Grollier G, Paute M, Desmaret M, Tanzer J . [Septicemia caused by Capnocytophaga sputigena]. Nouv Presse Med. 1981; 10(36):2992. View

18.

Coignard S, Malbrunot C, Bor Y, Perrot S, Rouveix E, Dechy H . [Endocarditis caused by capnocytophaga ochracea]. Nouv Presse Med. 1982; 11(17):1338. View

19.

Seger R, Kloeti J, von Graevenitz A, Wust J, Briner J, Willi U . Cervical abscess due to Capnocytophaga ochracea. Pediatr Infect Dis. 1982; 1(3):170-2. DOI: 10.1097/00006454-198205000-00009. View

20.

MASHIMO P, Yamamoto Y, Nakamura M, Slots J . Selective recovery of oral Capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin B. J Clin Microbiol. 1983; 17(2):187-91. PMC: 272606. DOI: 10.1128/jcm.17.2.187-191.1983. View