Ultrastructural Immunocytochemical Localization of Neuron-specific Enolase in Parafollicular Cells of the Rat Thyroid

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1985 Jan 1

PMID 4044303

Citations 2

Authors

M Zabel

H Schafer

Affiliations

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Abstract

Using pre- and post-embedding procedures, neuron-specific enolase and calcitonin were localized in rat thyroid parafollicular cells by light and electron microscopy. Peroxidase-antiperoxidase (PAP), biotin-avidin (ABC) and protein A--colloidal gold techniques were used. In paraffin sections neuron-specific enolase was demonstrated in all calcitonin-storing parafollicular cells in rats aging 1 to 180 days. The post-embedding procedure failed to detect neuron-specific enolase in ultrathin sections, but the enzyme could be demonstrated using a preembedding procedure. Neuron-specific enolase was localized exclusively within the cytosol of parafollicular cells, while calcitonin was localized within secretory granules applying either post- or pre-embedding incubation techniques.

Citing Articles

S-100 protein and neuron-specific enolase in parathyroid glands and C-cells of the thyroid.

Zabel M, Dietel M Histochemistry. 1987; 86(4):389-92.

PMID: 3570881 DOI: 10.1007/BF00494999.

Effect of hypercalcemia on parafollicular cells in the rat thyroid gland.

Zabel M, Schafer H Histochemistry. 1988; 88(3-6):623-8.

PMID: 2896647 DOI: 10.1007/BF00570333.

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