Experimentally Induced Defects of Mitochondrial Metabolism in Rat Skeletal Muscle. Biological Effects of the Mitochondrial Uncoupling Agent 2,4-dinitrophenol

Overview

Journal Biochem J

Specialty Biochemistry

Date 1985 Jul 1

PMID 4038252

Citations 5

Authors

E Byrne

D J Hayes

E A Shoubridge

J A Morgan-Hughes

J B Clark

Affiliations

Soon will be listed here.

Abstract

Infusion of dinitrophenol intra-arterially into rat hind limb caused an irreversible failure of isometric twitch tension and the induction of a severe progressive contracture. Metabolite analysis of muscle in which the twitch response had grossly fatigued revealed low levels of ATP and phosphocreatine together with lactate accumulation. Studies using 31P-n.m.r. confirmed the decrease in ATP and creatine phosphate concentrations and indicated a fall in intracellular pH. It is concluded that dinitrophenol-induced myopathy does not represent a good model for the human mitochondrial myopathic condition as has been previously suggested.

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