Muscle Denervation Increases the Levels of Two MRNAs Coding for the Acetylcholine Receptor Alpha-subunit

Overview

Journal J Neurosci

Specialty Neurology

Date 1985 Sep 1

PMID 4032011

Citations 30

Authors

D Goldman

J Boulter

S Heinemann

J Patrick

Affiliations

Soon will be listed here.

Abstract

The mRNA coding for the alpha-subunit of the acetylcholine receptor was studied in mouse leg and rat diaphragm muscle. We find that denervation of rat diaphragm results in a 7-fold increase in mRNA coding for the alpha-subunit, whereas denervation of mouse leg muscle results in approximately a 50-fold increase in alpha-subunit-specific mRNA. The relationship of the mRNAs purified from innervated and denervated muscle was investigated by SI nuclease mapping. Two mRNA species were found in both innervated and denervated muscle which differ in their 3'-untranslated region. The levels of both these mRNA species increase upon denervation of mouse leg muscle.

Citing Articles

Assessment of the expression and role of the α1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea.

Roux I, Wu J, McIntosh J, Glowatzki E J Neurophysiol. 2016; 116(2):479-92.

PMID: 27098031 PMC: 4978794. DOI: 10.1152/jn.01038.2015.

Ubiquitin homeostasis is critical for synaptic development and function.

Chen P, Bhattacharyya B, Hanna J, Minkel H, Wilson J, Finley D J Neurosci. 2011; 31(48):17505-13.

PMID: 22131412 PMC: 3253363. DOI: 10.1523/JNEUROSCI.2922-11.2011.

The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions.

Chen P, Qin L, Li X, Walters B, Wilson J, Mei L J Neurosci. 2009; 29(35):10909-19.

PMID: 19726649 PMC: 2766780. DOI: 10.1523/JNEUROSCI.2635-09.2009.

A histone deacetylase 4/myogenin positive feedback loop coordinates denervation-dependent gene induction and suppression.

Tang H, MacPherson P, Marvin M, Meadows E, Klein W, Yang X Mol Biol Cell. 2008; 20(4):1120-31.

PMID: 19109424 PMC: 2642751. DOI: 10.1091/mbc.e08-07-0759.

Human nicotinic acetylcholine receptor alpha-subunit isoforms: origins and expression.

MacLennan C, Beeson D, Vincent A, Newsom-Davis J Nucleic Acids Res. 1993; 21(23):5463-7.

PMID: 8265363 PMC: 310586. DOI: 10.1093/nar/21.23.5463.