Effects of Verapamil on Calcium-induced Rigidity and on Filterability of Red Blood Cells from Healthy Volunteers and Patients with Progressive Systemic Sclerosis

Overview

Journal Br J Clin Pharmacol

Specialty Pharmacology

Date 1985 Jun 1

PMID 4027116

Citations 5

Authors

S O Sowemimo-Coker

I B Kovacs

J D Kirby

P Turner

Affiliations

Soon will be listed here.

Abstract

The effects of verapamil on calcium-induced decrease deformability of red blood cells (RBCs) and on the filterability of RBCs from healthy volunteers and patients with progressive systemic sclerosis (PSS) were investigated in vitro using a gravity driven filtration technique. The filterability of RBCs was increased by verapamil 1 microgram/ml (P less than 0.01) in healthy volunteers (P less than 0.05) and in patients with PSS (P less than 0.05). A high concentration of verapamil (200 micrograms/ml) caused an 80% reduction (P less than 0.05) in the filterability of RBCs from healthy volunteers. The filterability of RBCs stored at 4 degrees C for 24 h was increased by 1 microgram/ml verapamil (P less than 0.05). Verapamil (1 microgram/ml) prevented the decrease in deformability of RBCs due to an increase in either extracellular or intracellular calcium concentrations (P less than 0.05). By increasing red cell filterability verapamil may be useful in the treatment of PSS and other peripheral vascular diseases where decreased red cell deformability may play an important role in the pathogenesis.

Citing Articles

Calcium supplementation during sepsis exacerbates organ failure and mortality via calcium/calmodulin-dependent protein kinase kinase signaling.

Collage R, Howell G, Zhang X, Stripay J, Lee J, Angus D Crit Care Med. 2013; 41(11):e352-60.

PMID: 23887235 PMC: 3812408. DOI: 10.1097/CCM.0b013e31828cf436.

Nisoldipine improves the impaired erythrocyte deformability correlating with elevated intracellular free calcium-ion concentration and poor glycaemic control in NIDDM.

Fujita J, Tsuda K, Takeda T, Yu L, Fujimoto S, Kajikawa M Br J Clin Pharmacol. 1999; 47(5):499-506.

PMID: 10336573 PMC: 2014185. DOI: 10.1046/j.1365-2125.1999.00934.x.

Trilinolein improves erythrocyte deformability during cardiopulmonary bypass.

Tsai S, Chan P, Lee T, Yung J, Hong C Br J Clin Pharmacol. 1994; 37(5):457-9.

PMID: 8054252 PMC: 1364902. DOI: 10.1111/j.1365-2125.1994.tb05714.x.

Clinical and rheological effects of nifedipine in Raynaud's phenomenon.

Waller D, Challenor V, Francis D, Roath O Br J Clin Pharmacol. 1986; 22(4):449-54.

PMID: 3533127 PMC: 1401145. DOI: 10.1111/j.1365-2125.1986.tb02916.x.

Ex vivo effects of nifedipine, nisoldipine and nitrendipine on filterability of red blood cells from healthy volunteers.

Sowemimo-Coker S, Debbas N, Kovacs I, Turner P Br J Clin Pharmacol. 1985; 20(2):152-4.

PMID: 2931096 PMC: 1400681. DOI: 10.1111/j.1365-2125.1985.tb05048.x.

References

Hoffman J . The red cell membrane and the transport of sodium and potassium. Am J Med. 1966; 41(5):666-80. DOI: 10.1016/0002-9343(66)90029-5. View

Nakao M, Nakao T, YAMAZOE S . Adenosine triphosphate and maintenance of shape of the human red cells. Nature. 1960; 187:945-6. DOI: 10.1038/187945a0. View

WEED R, Lacelle P, Merrill E . Metabolic dependence of red cell deformability. J Clin Invest. 1969; 48(5):795-809. PMC: 322288. DOI: 10.1172/JCI106038. View

SCHATZMANN H, VINCENZI F . Calcium movements across the membrane of human red cells. J Physiol. 1969; 201(2):369-95. PMC: 1351614. DOI: 10.1113/jphysiol.1969.sp008761. View

Lee K, Shin B . Studies on the active transport of calcium in human red cells. J Gen Physiol. 1969; 54(6):713-29. PMC: 2225953. DOI: 10.1085/jgp.54.6.713. View

Lichtman M, WEED R . Divalent cation content of normal and ATP-depleted erythrocytes and erythrocyte membranes. Nouv Rev Fr Hematol. 1972; 12(6):799-813. View

PALEK J, Stewart G, LIONETTI F . The dependence of shape of human erythrocyte ghosts on calcium, magnesium, and adenosine triphosphate. Blood. 1974; 44(4):583-97. View

FORTIER N, SNYDER L, PALEK J, Weiss E, Mancini C, Falcone J . Effect of propranolol on normal human erythrocytes. J Lab Clin Med. 1977; 89(1):41-50. View

Gardos G . The role of calcium in the potassium permeability of human erythrocytes. Acta Physiol Acad Sci Hung. 1959; 15(2):121-5. View

10.

Han P, Boatwright C, Ardlie N . Effect of the calcium-entry blocking agent nifedipine on activation of human platelets and comparison with verapamil. Thromb Haemost. 1983; 50(2):513-7. View

11.

Winston E, Pariser K, Miller K, Salem D, Creager M . Nifedipine as a therapeutic modality for Raynaud's phenomenon. Arthritis Rheum. 1983; 26(10):1177-80. DOI: 10.1002/art.1780261001. View

12.

Kovacs I, Sowemimo-Coker S, Kirby J, Turner P . Altered behaviour of erythrocytes in scleroderma. Clin Sci (Lond). 1983; 65(5):515-9. DOI: 10.1042/cs0650515. View

13.

Spearman T, Butcher F . The effect of calmodulin antagonists on amylase release from the rat parotid gland in vitro. Pflugers Arch. 1983; 397(3):220-4. DOI: 10.1007/BF00584361. View

14.

Kahan A, Weber S, Amor B, SAPORTA L, HODARA M, Degeorges M . Nifedipine and Raynaud's phenomenon. Ann Intern Med. 1981; 94(4 pt 1):546. DOI: 10.7326/0003-4819-94-4-546_1. View

15.

Burris S, Eaton J, White J . Evaluation of the role of diacyglycerol in calcium-induced erythrocyte shape change and rigidity. J Lab Clin Med. 1980; 96(4):749-56. View

16.

Woodcock B, Hopf R, Kaltenbach M . Verapamil and norverapamil plasma concentrations during long-term therapy in patients with hypertrophic obstructive cardiomyopathy. J Cardiovasc Pharmacol. 1980; 2(1):17-23. DOI: 10.1097/00005344-198001000-00003. View

17.

Eaton J, JACOB H, White J . Membrane abnormalities of irreversibly sickled cells. Semin Hematol. 1979; 16(1):52-64. View

18.

PALEK J . Red cell membrane injury in sickle cell anaemia. Br J Haematol. 1977; 35(1):1-9. DOI: 10.1111/j.1365-2141.1977.tb00556.x. View

19.

Olson E, CAZORT R . Active calcium and strontium transport in human erythrocyte ghosts. J Gen Physiol. 1969; 53(3):311-22. PMC: 2202910. DOI: 10.1085/jgp.53.3.311. View