Some Properties of a Membrane-deoxyribonucleic Acid Complex Isolated from Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1977 Feb 1

PMID 402358

Citations 5

Authors

J M HARMON

H W TABER

Affiliations

Soon will be listed here.

Abstract

Membrane-deoxyribonucleic acid (DNA) complexes were isolated from Bacillus subtilis by affinity for magnesium-Sarkosyl crystals. These complexes (M-bands) contained greater than 80% of the total cellular DNA; little of the remaining portion could be recovered in a secondary isolation. Isotopic labeling of the origin of replication showed this region of the chromosome to be closely associated with the cell membrane. Interruption of protein or DNA synthesis did not result in detachment of the chromosome from the membrane. Interruption of ribonucleic acid synthesis by rifampin resulted in a decreased ability to isolate DNA in the M-band. Analysis of attachment of the chromosome to membrane during the cell and replication cycles indicated that the chromosome is not released from the membrane at any time during the cell cycle.

Citing Articles

Interaction of polynucleotides with natural and model membranes.

Budker V, Godovikov A, NAUMOVA L, Slepneva I Nucleic Acids Res. 1980; 8(11):2499-515.

PMID: 6160462 PMC: 324097. DOI: 10.1093/nar/8.11.2499.

Membrane particles from Escherichia coli and Bacillus subtilis, containing penicillin-binding proteins and enriched for chromosomal-origin DNA.

Bone E, Todd J, Ellar D, Sargent M, Wyke A J Bacteriol. 1985; 164(1):192-200.

PMID: 3930465 PMC: 214229. DOI: 10.1128/jb.164.1.192-200.1985.

Altered accumulation of a membrane protein unique to a membrane-deoxyribonucleic acid complex in a dna initiation mutant of Bacillus subtilis.

HARMON J, TABER H J Bacteriol. 1977; 130(3):1224-33.

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Association of the folded chromosome with the cell envelope of Escherichia coli: nature of the membrane-associated DNA.

Drlica K, Burgi E, WORCEL A J Bacteriol. 1978; 134(3):1108-16.

PMID: 350839 PMC: 222361. DOI: 10.1128/jb.134.3.1108-1116.1978.

Benzeneboronic acid selectively inhibits sporulation of Bacillis subtilis.

Lopez I, Hageman J J Bacteriol. 1978; 136(2):625-30.

PMID: 30755 PMC: 218587. DOI: 10.1128/jb.136.2.625-630.1978.

References

Anagnostopoulos C, Spizizen J . REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961; 81(5):741-6. PMC: 279084. DOI: 10.1128/jb.81.5.741-746.1961. View

RYTER A, Jacob F . [ELECTRON MICROSCOPE STUDY OF THE LINKAGE BETWEEN NUCLEUS AND MESOSOME IN BACILLUS SUBTILIS]. Ann Inst Pasteur (Paris). 1964; 107:384-400. View

Yamaguchi K, Yoshikawa H . Topography of chromosome membrane junction in Bacillus subtilis. Nat New Biol. 1973; 244(137):204-6. DOI: 10.1038/newbio244204a0. View

Jones N, DONACHIE W . Protein synthesis and the release of the replicated chromosome from the cell membrane. Nature. 1974; 251(5472):252-4. DOI: 10.1038/251252a0. View

Dworsky P, Schaechter M . Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli. J Bacteriol. 1973; 116(3):1364-74. PMC: 246496. DOI: 10.1128/jb.116.3.1364-1374.1973. View

Villani G, Mastromei G, Riva S . Membrane attachment of the chromosome in Bacillus subtilis mutants temperature-sensitive in DNA replication. Nucleic Acids Res. 1974; 1(12):1775-82. PMC: 343456. DOI: 10.1093/nar/1.12.1775. View

Mendelson N . Deoxyribonucleic acid distribution in Bacillus subtilis independent of cell elongation. J Bacteriol. 1972; 111(1):156-62. PMC: 251252. DOI: 10.1128/jb.111.1.156-162.1972. View

OSULLIVAN M, Sueoka N . Membrane attachment of the replication origins of a multifork (dichotomous) chromosome in Bacillus subtilis. J Mol Biol. 1972; 69(2):237-48. DOI: 10.1016/0022-2836(72)90228-8. View

Ivarie R, PENE J . Association of many regions of the Bacillus subtilis chromosome with the cell membrane. J Bacteriol. 1973; 114(2):571-6. PMC: 251811. DOI: 10.1128/jb.114.2.571-576.1973. View

10.

WORCEL A, Burgi E . Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol. 1974; 82(1):91-105. DOI: 10.1016/0022-2836(74)90576-2. View

11.

PETTIJOHN D, Hecht R . RNA molecules bound to the folded bacterial genome stabilize DNA folds and segregate domains of supercoiling. Cold Spring Harb Symp Quant Biol. 1974; 38:31-41. DOI: 10.1101/sqb.1974.038.01.006. View

12.

Olsen W, HEIDRICH H, Hannig K, Hofschneider P . Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization. J Bacteriol. 1974; 118(2):646-53. PMC: 246800. DOI: 10.1128/jb.118.2.646-653.1974. View

13.

Ivarie R, PENE J . Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients. J Bacteriol. 1970; 104(2):839-50. PMC: 285067. DOI: 10.1128/jb.104.2.839-850.1970. View

14.

Sueoka N, Quinn W . Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968; 33:695-705. DOI: 10.1101/sqb.1968.033.01.078. View

15.

TREMBLAY G, Daniels M, Schaechter M . Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969; 40(1):65-76. DOI: 10.1016/0022-2836(69)90296-4. View

16.

CHATTERJEE A, Taber H, Young F . A rapid method for synchronization of Staphylococcus aureus and Bacillus subtilis. Biochem Biophys Res Commun. 1971; 44(5):1125-30. DOI: 10.1016/s0006-291x(71)80202-4. View

17.

Fielding P, Fox C . Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli. Biochem Biophys Res Commun. 1970; 41(1):157-62. DOI: 10.1016/0006-291x(70)90482-1. View

18.

Smith D, HANAWALT P . Properties of the growing point region in the bacterial chromosome. Biochim Biophys Acta. 1967; 149(2):519-31. DOI: 10.1016/0005-2787(67)90180-3. View

19.

Bettinger G, Young F . Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts. J Bacteriol. 1975; 122(3):987-93. PMC: 246150. DOI: 10.1128/jb.122.3.987-993.1975. View

20.

Yamaguchi K, Yoshikawa H . Association of the replication terminus of the Bacillus subtilis chromosome to the cell membrane. J Bacteriol. 1975; 124(2):1030-3. PMC: 235995. DOI: 10.1128/jb.124.2.1030-1033.1975. View