(6-4)Photoproducts Are Removed from the DNA of UV-irradiated Mammalian Cells More Efficiently Than Cyclobutane Pyrimidine Dimers

Overview

Journal Mutat Res

Publisher Elsevier

Specialty Genetics

Date 1985 Jul 1

PMID 4010689

Citations 65

Authors

D L Mitchell

C A Haipek

J M Clarkson

Affiliations

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Abstract

A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.

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