The Aldehyde Dehydrogenase Superfamily in L.: Genome-Wide Identification and Expression Analysis Under Low-Temperature Conditions

The Aldehyde Dehydrogenase (ALDH) superfamily comprises a group of NAD or NADP-dependent enzymes that play essential roles in responding to abiotic stresses in plants. In L., however, the increasing frequency of extremely low temperatures during winter in recent years has significantly affected both yield and quality. This study conducted a genome-wide screening of superfamily genes, analyzing their gene structures, evolutionary relationships, protein physicochemical properties, and expression patterns under low-temperature stress to explore the function of the superfamily gene in cold tolerance in L. A total of six genes with significant differences in expression levels were verified utilizing quantitative real-time polymerase chain reaction (qRT-PCR), revealing that , , , , , and all exhibited higher expression in cold-tolerant material 24W233 compared with cold-sensitive material 24W259. Additionally, a single nucleotide polymorphism (SNP) in the promoter region shows differences between the cold-tolerant (24W233) and the cold-sensitive (24W259) varieties, and it may be associated with the cold tolerance of these two varieties. This comprehensive analysis offers valuable insights into the role of family genes in low-temperature stress adaptation in and offers genetic resources for the development of novel cold-tolerant cultivars.