Comparison of Isolated from Human Clinical Cases and Cat Carriers Regarding Antibiotic Susceptibility and Biofilm Production

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2025 Mar 13

PMID 40076573

Authors

Karolina Bierowiec

Ashley Delmar

Magdalena Karwanska

Magdalena Siedlecka

Aleksandra Kumala-Cwikla

Marta Ksiazczyk

Katarzyna Kapczynska

Affiliations

Soon will be listed here.

Abstract

is a rare opportunistic bacterium not commonly found in healthy individuals or animals has increasing clinical significance in both veterinary and human medicine due to its multidrug resistance and biofilm-forming ability. This study analyzed 12 isolates of collected from humans and cats and identified them using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and and partial B gene sequencing. All of the were phenotypically resistant to penicillin, and almost all (except one human strain) were resistant to methicillin. Antibiotic susceptibility testing revealed a high prevalence of multidrug resistance in all human strains with frequent resistance to β-lactams, macrolides, and tetracyclines. A comparative analysis of human and feline isolates indicated the presence of shared resistance genes such as , , and . Biofilm production varied across isolates, with more potent biofilm formation abilities observed at elevated temperatures (39 °C) and time (48 h). These findings underscore the potential zoonotic risks of and its role in managing multidrug-resistant infections.

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