Regulates the Proliferation and Apoptosis of Ovarian Granulosa Cells by Targeting in Zhedong White Geese

Overview

Journal Animals (Basel)

Date 2025 Mar 13

PMID 40075946

Authors

Size Wang

Chuicheng Zeng

Yue Pan

Zhengyu Zang

Yuanliang Zhang

Shan Yue

Xiuhua Zhao

He Huang

Affiliations

Soon will be listed here.

Abstract

The proliferation and apoptosis of ovarian granulosa cells (GC) is critical for follicular development and ovulation, especially for egg-laying performance in female birds. In geese, follicular atrophy is accompanied by follicular GC apoptosis during brooding stages. MicroRNAs are involved in follicular development, atrophy, ovulation, and degeneration. Our previous high-throughput sequencing study of ovaries from laying and brooding geese revealed that may be involved in follicle growth and development, as well as follicle cell proliferation and apoptosis in geese. To further investigate the effect of on GC in geese, we screened the target gene of () and constructed a overexpression and interference vector, synthesized a small interfering RNA for . The results showed that after overexpression, the mRNA and protein expression of proliferation-related genes (, , , and ) were significantly decreased, the mRNA and protein expression of apoptosis-related genes (, , and ) were significantly increased, and the mRNA and protein expression of anti-apoptosis gene were significantly decreased. While the mRNA and protein expressions of , , , and were significantly decreased after the downregulation of expression, the mRNA and protein expressions of , , and were significantly increased, and the mRNA and protein expression of was significantly decreased. In conclusion, inhibited proliferation and promoted apoptosis of follicular GC by targeting in Zhedong White geese. Apoptosis of GC leads to follicular atresia, which in turn leads to brooding behavior in female geese. Therefore, it is possible to explore inhibiting the expression of in production to alter the brooding behavior of Zhedong White Geese, thereby improving egg production and economic returns for producers.

References

Li T, Forbes M, Fuller G, Li J, Yang X, Zhang W . IGFBP2: integrative hub of developmental and oncogenic signaling network. Oncogene. 2020; 39(11):2243-2257. PMC: 7347283. DOI: 10.1038/s41388-020-1154-2. View

Khan S, Lu X, Huang Q, Tang J, Weng J, Yang Z . IGFBP2 Plays an Essential Role in Cognitive Development during Early Life. Adv Sci (Weinh). 2019; 6(23):1901152. PMC: 6891907. DOI: 10.1002/advs.201901152. View

Zhang J, Xu Y, Liu H, Pan Z . MicroRNAs in ovarian follicular atresia and granulosa cell apoptosis. Reprod Biol Endocrinol. 2019; 17(1):9. PMC: 6329178. DOI: 10.1186/s12958-018-0450-y. View

Li F, Xing Y, Zhang J, Mu J, Ge J, Zhao M . Goose Hepatic IGFBP2 Is Regulated by Nutritional Status and Participates in Energy Metabolism Mainly through the Cytokine-Cytokine Receptor Pathway. Animals (Basel). 2023; 13(14). PMC: 10376900. DOI: 10.3390/ani13142336. View

Wang Z, Zhang X, Li Z, Abdalla B, Chen Y, Nie Q . MiR-34b-5p Mediates the Proliferation and Differentiation of Myoblasts by Targeting IGFBP2. Cells. 2019; 8(4). PMC: 6523632. DOI: 10.3390/cells8040360. View

Wang C, Sun H, Davis J, Wang X, Huo L, Sun N . FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells. Cell Death Dis. 2023; 14(4):239. PMC: 10073124. DOI: 10.1038/s41419-023-05759-3. View

Park S, Kim K, Kim J . The Role of Insulin-Like Growth Factor Binding Protein 2 (IGFBP2) in the Regulation of Corneal Fibroblast Differentiation. Invest Ophthalmol Vis Sci. 2015; 56(12):7293-302. DOI: 10.1167/iovs.15-16616. View

Wei C, Xiang S, Yu Y, Song J, Zheng M, Lian F . miR-221-3p regulates apoptosis of ovarian granulosa cells via targeting FOXO1 in older women with diminished ovarian reserve (DOR). Mol Reprod Dev. 2021; 88(4):251-260. PMC: 8251591. DOI: 10.1002/mrd.23457. View

Ding H, Wu T . Insulin-Like Growth Factor Binding Proteins in Autoimmune Diseases. Front Endocrinol (Lausanne). 2018; 9:499. PMC: 6125368. DOI: 10.3389/fendo.2018.00499. View

10.

Slater T, Haywood N, Matthews C, Cheema H, Wheatcroft S . Insulin-like growth factor binding proteins and angiogenesis: from cancer to cardiovascular disease. Cytokine Growth Factor Rev. 2019; 46:28-35. DOI: 10.1016/j.cytogfr.2019.03.005. View

11.

Grodowitz M, Reed D, Elliott B, Perring T . Female Reproductive System Morphology and the Development of a Physiological Age-Grading System for Bagrada hilaris (Hemiptera: Pentatomidae). J Insect Sci. 2019; 19(2). PMC: 6417153. DOI: 10.1093/jisesa/iez022. View

12.

Erceylan O, Savas A, Gov E . Targeting the tumor stroma: integrative analysis reveal GATA2 and TORYAIP1 as novel prognostic targets in breast and ovarian cancer. Turk J Biol. 2021; 45(2):127-137. PMC: 8068767. DOI: 10.3906/biy-2010-39. View

13.

Zhang J, Ren Q, Chen J, Lv L, Wang J, Shen M . Downregulation of miR-192 Alleviates Oxidative Stress-Induced Porcine Granulosa Cell Injury by Directly Targeting Acvr2a. Cells. 2022; 11(15). PMC: 9368079. DOI: 10.3390/cells11152362. View

14.

Hu L, Li D, Wei Q, Kang L, Sun Y, Jiang Y . Characterization of a novel IGFBP-2 transcript in the ovarian granulosa cells of chicken follicles: mRNA expression, function and effect of reproductive hormones and IGF1. Poult Sci. 2024; 103(12):104501. PMC: 11577207. DOI: 10.1016/j.psj.2024.104501. View

15.

Wu Y, Huang Y, Chang T, Chen C, Wu P, Huang S . COL11A1 activates cancer-associated fibroblasts by modulating TGF-β3 through the NF-κB/IGFBP2 axis in ovarian cancer cells. Oncogene. 2021; 40(26):4503-4519. DOI: 10.1038/s41388-021-01865-8. View

16.

Kunhiraman H, McSwain L, Shahab S, Gershon T, MacDonald T, Kenney A . IGFBP2 promotes proliferation and cell migration through STAT3 signaling in Sonic hedgehog medulloblastoma. Acta Neuropathol Commun. 2023; 11(1):62. PMC: 10082504. DOI: 10.1186/s40478-023-01557-2. View

17.

Han S, Wang J, Cui C, Yu C, Zhang Y, Li D . Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken. Poult Sci. 2021; 101(1):101524. PMC: 8591502. DOI: 10.1016/j.psj.2021.101524. View

18.

Lee E, Mircean C, Shmulevich I, Wang H, Liu J, Niemisto A . Insulin-like growth factor binding protein 2 promotes ovarian cancer cell invasion. Mol Cancer. 2005; 4(1):7. PMC: 549074. DOI: 10.1186/1476-4598-4-7. View

19.

Khristi V, Chakravarthi V, Singh P, Ghosh S, Pramanik A, Ratri A . ESR2 regulates granulosa cell genes essential for follicle maturation and ovulation. Mol Cell Endocrinol. 2018; 474:214-226. DOI: 10.1016/j.mce.2018.03.012. View

20.

Zhang B, Chen L, Feng G, Xiang W, Zhang K, Chu M . MicroRNA Mediating Networks in Granulosa Cells Associated with Ovarian Follicular Development. Biomed Res Int. 2017; 2017:4585213. PMC: 5337806. DOI: 10.1155/2017/4585213. View