Advances in Novel Diagnostic Techniques for Alveolar Echinococcosis

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2025 Mar 13

PMID 40075832

Authors

Huanhuan Liu

Yijia Xie

Xiaoyu An

Dazhuang Xu

Shundong Cai

Chengchao Chu

Gang Liu

Affiliations

Soon will be listed here.

Abstract

Alveolar echinococcosis (AE), caused by the larval stage of the tapeworm , is a serious parasitic disease that presents significant health risks and challenges for both patients and healthcare systems. Accurate and timely diagnosis is essential for effective management and improved patient outcomes. This review summarizes the latest diagnostic methods for AE, focusing on serological tests and imaging techniques such as ultrasonography (US), computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography/computed tomography (PET/CT). Each imaging modality has its strengths and limitations in detecting and characterizing AE lesions, such as their location, size, and invasiveness. US is often the first-line method due to its non-invasiveness and cost-effectiveness, but it may have limitations in assessing complex lesions. CT provides detailed anatomical information and is particularly useful for assessing bone involvement and calcification. MRI, with its excellent soft tissue contrast, is superior for delineating the extent of AE lesions and their relationship to adjacent structures. PET/CT combines functional and morphological imaging to provide insights into the metabolic activity of lesions, which is valuable for monitoring treatment response and detecting recurrence. Overall, this review emphasizes the importance of a multifaceted diagnostic approach that combines serological and imaging techniques for accurate and early AE diagnosis, which is crucial for effective management and improved patient outcomes.

References

Ma L, Liu W, Mao B, Zhang Y . An Echinococcosis multilocularis presenting as a giant anterior cranial basilar tumor. Neurol Sci. 2009; 30(3):247-9. DOI: 10.1007/s10072-009-0042-x. View

Bulakci M, Ilhan M, Bademler S, Yilmaz E, Gulluoglu M, Bayraktar A . Efficacy of ultrasound-guided core-needle biopsy in the diagnosis of hepatic alveolar echinococcosis: a retrospective analysis. Parasite. 2016; 23:19. PMC: 4840258. DOI: 10.1051/parasite/2016019. View

Torgerson P, Keller K, Magnotta M, Ragland N . The global burden of alveolar echinococcosis. PLoS Negl Trop Dis. 2010; 4(6):e722. PMC: 2889826. DOI: 10.1371/journal.pntd.0000722. View

Ito A, Xiao N, Liance M, Sato M, Sako Y, Mamuti W . Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test. J Clin Microbiol. 2002; 40(11):4161-5. PMC: 139688. DOI: 10.1128/JCM.40.11.4161-4165.2002. View

Liu C, Bi X, Fan H, Ma L, Ge R . Microcyst fluid promotes the migration and invasion of fibroblasts in the adventitial layer of alveolar echinococcosis. Acta Trop. 2021; 223:106084. DOI: 10.1016/j.actatropica.2021.106084. View

Bi X, Shao Y, Li L, Wang Y, Zhang C, Lu G . Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China. Exp Ther Med. 2018; 16(4):3155-3160. PMC: 6125881. DOI: 10.3892/etm.2018.6555. View

Kronenberg P, Reinehr M, Eichenberger R, Hasler S, Laurimae T, Weber A . Monoclonal antibody-based localization of major diagnostic antigens in metacestode tissue, excretory/secretory products, and extracellular vesicles of species. Front Cell Infect Microbiol. 2023; 13:1162530. PMC: 10061086. DOI: 10.3389/fcimb.2023.1162530. View

Eberhardt N, Peters L, Kapp-Schwoerer S, Beer M, Beer A, Gruner B . 18F-FDG-PET/MR in Alveolar Echinococcosis: Multiparametric Imaging in a Real-World Setting. Pathogens. 2022; 11(3). PMC: 8951377. DOI: 10.3390/pathogens11030348. View

Carmena D, Benito A, Eraso E . The immunodiagnosis of Echinococcus multilocularis infection. Clin Microbiol Infect. 2007; 13(5):460-75. DOI: 10.1111/j.1469-0691.2007.01665.x. View

10.

Demirkazik M, Koltas I, Inceboz T, Korkmaz M, Gumurdulu D . Use of the ELISA (Em2-Em18) and Western Blotting Methods on Diagnosis of Alveolar Echinococcosis. Turkiye Parazitol Derg. 2019; 43(Suppl 1):13-17. DOI: 10.4274/tpd.galenos.2019.6447. View

11.

Faucher J, Descotes-Genon C, Hoen B, Godard J, Felix S, Aubry S . Hints for control of infection in unique extrahepatic vertebral alveolar echinococcosis. Infection. 2016; 45(3):365-368. DOI: 10.1007/s15010-016-0974-z. View

12.

Brehm K, Koziol U . Echinococcus-Host Interactions at Cellular and Molecular Levels. Adv Parasitol. 2017; 95:147-212. DOI: 10.1016/bs.apar.2016.09.001. View

13.

Claudon M, Dietrich C, Choi B, Cosgrove D, Kudo M, Nolsoe C . Guidelines and good clinical practice recommendations for Contrast Enhanced Ultrasound (CEUS) in the liver - update 2012: A WFUMB-EFSUMB initiative in cooperation with representatives of AFSUMB, AIUM, ASUM, FLAUS and ICUS. Ultrasound Med Biol. 2012; 39(2):187-210. DOI: 10.1016/j.ultrasmedbio.2012.09.002. View

14.

Barth T, Herrmann T, Tappe D, Stark L, Gruner B, Buttenschoen K . Sensitive and specific immunohistochemical diagnosis of human alveolar echinococcosis with the monoclonal antibody Em2G11. PLoS Negl Trop Dis. 2012; 6(10):e1877. PMC: 3493387. DOI: 10.1371/journal.pntd.0001877. View

15.

Harman M, Arslan H, Kotan C, Etlik O, Kayan M, Deveci A . MRI findings of hepatic alveolar echinococcosis. Clin Imaging. 2003; 27(6):411-6. DOI: 10.1016/s0899-7071(03)00006-8. View

16.

Asanuma T, Kawahara T, Inanami O, Nakao M, Nakaya K, Ito A . Magnetic resonance imaging of alveolar echinococcosis experimentally induced in the rat lung. J Vet Med Sci. 2006; 68(1):15-20. DOI: 10.1292/jvms.68.15. View

17.

Dietrich C, Douira-Khomsi W, Gharbi H, Sharma M, Cui X, Sparchez Z . Cystic and alveolar echinococcosis of the hepatobiliary tract - the role of new imaging techniques for improved diagnosis. Med Ultrason. 2020; 22(1):75-84. DOI: 10.11152/mu-2421. View

18.

Spahn S, Helmchen B, Zingg U . Alveolar echinococcosis of the right adrenal gland: a case report and review of the literature. J Med Case Rep. 2016; 10(1):325. PMC: 5111185. DOI: 10.1186/s13256-016-1115-0. View

19.

Zeng H, Wang J, Xie W, Liu W, Wen H . Assessment of early hepatic echinococcus multilocularis infection in rats with real-time contrast-enhanced ultrasonography. Ultrasound Med Biol. 2012; 38(11):1982-8. DOI: 10.1016/j.ultrasmedbio.2012.07.007. View

20.

Chen L, Cheng Z, Xian S, Zhan B, Xu Z, Yan Y . Immunization with CRT-Induced Protective Immunity against Infection in BALB/c Mice. Trop Med Infect Dis. 2022; 7(10). PMC: 9610995. DOI: 10.3390/tropicalmed7100279. View