Description of Cytobacillus Mangrovibacter Sp. Nov., and Cytobacillus Spartinae Sp. Nov., Isolated from Mangrove Sediment

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2025 Mar 12

PMID 40069467

Authors

Huai Shi

Manik Prabhu Narsing Rao

Qian-Qian Chen

Syed Raziuddin Quadri

Wen-Jun Li

Guo-Hong Liu

Affiliations

Soon will be listed here.

Abstract

Two Gram-stain-positive, aerobic, rod-shaped, and motile strains FJAT-53684 and FJAT-54145 were isolated from the mangrove sediment. They optimally grew at pH 8.0 and could tolerate NaCl up to 5% (w/v). The optimum temperature for growth of strains FJAT-53684 and FJAT-54145 were 35 and 40 ºC, respectively. The 16S rRNA gene sequence similarity between FJAT-53684 and FJAT-54145 was 97.1%. Both strains showed the highest 16S rRNA gene sequence similarity to the Cytobacillus species. Menaquinone-7 was the only respiratory quinone present in both strains. The major fatty acids (≥ 10%) in strain FJAT-53684 were iso-C, anteiso-C, iso-C, and iso-C, while strain FJAT-54145 consist of iso-C, iso-C, iso-C, iso-C, and C ω7c alcohol. The polar lipids present in both strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified lipid, unidentified phospholipid, and phosphatidylinositol. Their average nucleotide identity and digital DNA-DNA hybridization values were below the threshold values (95% and 70%, respectively) for species delineation. Based on the above results, strains FJAT-53684 and FJAT-54145 represent two novel species of the genus Cytobacillus, for which the names Cytobacillus mangrovibacter sp. nov., and Cytobacillus spartinae sp. nov., are proposed. The type strains are FJAT-53684 (= JCM 35618 = GDMCC 1.3071), and FJAT-54145 (= JCM 35621 = GDMCC 1.3079).

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