Development of a Polymicrobial Host-bacterial Interface Biofilm Model for Bacterial Vaginosis

Bacterial vaginosis (BV) is characterised by a polymicrobial biofilm forming on the vaginal epithelium. In this study, we have developed a host-pathogen model of BV to replicate disease. We demonstrated tissue colonisation by four key vaginal pathobionts that formed metronidazole tolerant biofilms, with subtle changes in cytotoxicity and inflammation.