Expression of a Modified Avr3a Gene Under the Control of a Synthetic Pathogen-inducible Promoter Leads to Phytophthora Infestans Resistance in Potato

Late blight resistance of potato was improved by the co-expression of the potato resistance gene R3a and the pathogen-inducible avirulence gene Avr3a of Phytopthora infestans. The synthetic pathogen-inducible promoter 2xS-4xD-NpCABE, which is composed of the cis-acting elements S and D and the core promoter of the NpCABE gene, was developed for potato. By analysis of 20 core promoters from Solanacea species synthetic promoters of the 2xS-2xD-type were generated which differ in their background activity, strength and promoter inducibility. These data showed that the core promoter plays an important role for the architecture of a synthetic promoter and influences the specificity and strength beside the cis-acting element. The 2xS-2xD-NpCABE promoter was further improved by increasing the number of the cis-acting elements resulting in the 2xS-4xD-NpCABE promoter. Modified Avr3a alleles, which triggered less cell death than the Avr3a allele, were expressed with the optimized synthetic promoter in transgenic potatoes with an R3a gene. The transgenic lines showed less late blight symptoms and up to 60% reduction of sporangia in detached leaf assays. The absence of a negative plant phenotype in the greenhouse demonstrated that the balanced co-expression of a modified Avr3a gene under the control of an optimized synthetic promoter is a promising strategy to increase late blight resistance of potatoes. This concept might be as well applied to other crops since the co-expression of the R3a and Avr3a gene induced cell death in leaves of corn, wheat and soybean in a transient assay.