LncRNA-MALAT1 Promotes Triple-negative Breast Cancer Progression and Function As CeRNA to Target REEP5 by Sponging MiR-106a-5p

Overview

Journal Eur J Med Res

Publisher Biomed Central

Specialty General Medicine

Date 2025 Mar 10

PMID 40059235

Authors

Qiu-Hui Yang

Ye-Qin Fu

Wei-Liang Feng

Jie-Fei Mao

Ning Xu

Qing Liu

Qian-Jun Yan

Hong-Jian Yang

Xi-Ping Zhang

Affiliations

Soon will be listed here.

Abstract

Axillary lymph node metastasis (ALNM) in triple negative breast cancer (TNBC) will lead to poor prognosis. Recent studies have shown that long non-coding RNAs (lncRNAs) were involved in the progression of tumors. This study aimed to explore the role and mechanism of lncRNA-MALAT1 in the progression of TNBC and its relationship with ALNM. MALAT1 is highly expressed in TNBC cells lines, tumor tissues and serum, and it is positively correlated with the degree of ALNM. In addition, MALAT1 can act as a competitive endogenous RNA (ceRNA) that regulates cellular biological behavior by competitively binding to miR-106a-5p with REEP5. In conclusion, our results show that MALAT1 could function as ceRNA promote the proliferation, invasion and metastasis of TNBC cells through MALAT1/miR-106a-5p/REEP5 axis, which is expected to provide new ideas for the diagnosis of TNBC in clinic.

References

Yao L, Xie D, Geng L, Shi D, Huang J, Wu Y . REEP5 (Receptor Accessory Protein 5) Acts as a Sarcoplasmic Reticulum Membrane Sculptor to Modulate Cardiac Function. J Am Heart Assoc. 2018; 7(3). PMC: 5850239. DOI: 10.1161/JAHA.117.007205. View

Liu S, Teng F, Lu Y, Zhu Y, Liang X, Wu F . Ethoxy-erianin phosphate inhibits angiogenesis in colorectal cancer by regulating the TMPO-AS1/miR-126-3p/PIK3R2 axis and inactivating the PI3k/AKT signaling pathway. BMC Cancer. 2024; 24(1):1275. PMC: 11476319. DOI: 10.1186/s12885-024-12893-4. View

Fan S, Liu H, Li L . The REEP family of proteins: Molecular targets and role in pathophysiology. Pharmacol Res. 2022; 185:106477. DOI: 10.1016/j.phrs.2022.106477. View

Ha H, Debnath B, Neamati N . Role of the CXCL8-CXCR1/2 Axis in Cancer and Inflammatory Diseases. Theranostics. 2017; 7(6):1543-1588. PMC: 5436513. DOI: 10.7150/thno.15625. View

Liang Y, Li J, Yuan Y, Ju H, Liao H, Li M . Exosomal miR-106a-5p from highly metastatic colorectal cancer cells drives liver metastasis by inducing macrophage M2 polarization in the tumor microenvironment. J Exp Clin Cancer Res. 2024; 43(1):281. PMC: 11462797. DOI: 10.1186/s13046-024-03204-7. View

Wang B, Shen Y, Liu T, Tan L . ERα promotes transcription of tumor suppressor gene by establishing H3K27ac-enriched chromatin microenvironment in breast cancer cells. J Zhejiang Univ Sci B. 2021; 22(12):1034-1044. PMC: 8669322. DOI: 10.1631/jzus.B2100393. View

Lee S, Hadipour-Lakmehsari S, Murthy H, Gibb N, Miyake T, Teng A . REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects. Nat Commun. 2020; 11(1):965. PMC: 7031342. DOI: 10.1038/s41467-019-14143-9. View

Zhang H, Ge Z, Wang Z, Gao Y, Wang Y, Qu X . Circular RNA RHOT1 promotes progression and inhibits ferroptosis via mir-106a-5p/STAT3 axis in breast cancer. Aging (Albany NY). 2021; 13(6):8115-8126. PMC: 8034942. DOI: 10.18632/aging.202608. View

Zheng Y, Zhu K, Wang G . miR-106a-5p carried by tumor-derived extracellular vesicles promotes the invasion and metastasis of ovarian cancer by targeting KLF6. Clin Exp Metastasis. 2022; 39(4):603-621. DOI: 10.1007/s10585-022-10165-8. View

10.

Sun J, Li X, Yu E, Liu J, Sun L, He Q . A novel tumor suppressor ASMTL-AS1 regulates the miR-1228-3p/SOX17/β-catenin axis in triple-negative breast cancer. Diagn Pathol. 2021; 16(1):45. PMC: 8130432. DOI: 10.1186/s13000-021-01105-3. View

11.

Wu S, Lu J, Zhu H, Wu F, Mo Y, Xie L . A novel axis of circKIF4A-miR-637-STAT3 promotes brain metastasis in triple-negative breast cancer. Cancer Lett. 2023; 581:216508. DOI: 10.1016/j.canlet.2023.216508. View

12.

Yan H, Gao S, Xu A, Zuo L, Zhang J, Zhao Y . MALAT1 regulates network of microRNA-15a/16-VEGFA to promote tumorigenesis and angiogenesis in multiple myeloma. Carcinogenesis. 2023; 44(10-11):760-772. DOI: 10.1093/carcin/bgad053. View

13.

Wu J, Li M, Zhang Y . Long noncoding RNA HOXA-AS2 regulates the expression of SCN3A by sponging miR-106a in breast cancer. J Cell Biochem. 2019; 120(9):14465-14475. DOI: 10.1002/jcb.28706. View

14.

Chen F, Yan B, Ren J, Lyu R, Wu Y, Guo Y . FIT2 organizes lipid droplet biogenesis with ER tubule-forming proteins and septins. J Cell Biol. 2021; 220(5). PMC: 8056755. DOI: 10.1083/jcb.201907183. View

15.

Bray F, Laversanne M, Sung H, Ferlay J, Siegel R, Soerjomataram I . Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024; 74(3):229-263. DOI: 10.3322/caac.21834. View

16.

Bridges M, Daulagala A, Kourtidis A . LNCcation: lncRNA localization and function. J Cell Biol. 2021; 220(2). PMC: 7816648. DOI: 10.1083/jcb.202009045. View

17.

Yang S, Wang X, Zhou X, Hou L, Wu J, Zhang W . ncRNA-mediated ceRNA regulatory network: Transcriptomic insights into breast cancer progression and treatment strategies. Biomed Pharmacother. 2023; 162:114698. DOI: 10.1016/j.biopha.2023.114698. View

18.

Kim T, Croce C . MicroRNA and ER stress in cancer. Semin Cancer Biol. 2021; 75:3-14. DOI: 10.1016/j.semcancer.2020.12.025. View

19.

Wang N, Clark L, Gao Y, Kozlov M, Shemesh T, Rapoport T . Mechanism of membrane-curvature generation by ER-tubule shaping proteins. Nat Commun. 2021; 12(1):568. PMC: 7835363. DOI: 10.1038/s41467-020-20625-y. View

20.

Coan M, Haefliger S, Ounzain S, Johnson R . Targeting and engineering long non-coding RNAs for cancer therapy. Nat Rev Genet. 2024; 25(8):578-595. DOI: 10.1038/s41576-024-00693-2. View