The Genome Sequence of the Kissing Bug, (Latreille, 1811)

Overview

Journal Wellcome Open Res

Specialty Biomedical Engineering

Date 2025 Mar 5

PMID 40041610

Authors

Cristian Canizales-Silva

Mateo A Alvarado-Lopez

Carolina Hernandez

Carlos Ospina

Gustavo A Vallejo

Martin S Llewellyn

Juan David Ramirez

Affiliations

Soon will be listed here.

Abstract

We present a genome assembly from an individual female (kissing bug; Arthropoda; Insecta; Hemiptera; Reduviidae). The assembly contains two haplotypes with total lengths of 1,362.73 megabases and 1,342.40 megabases, respectively. Most of haplotype 1 (97.5%) is scaffolded into 12 chromosomal pseudomolecules. Haplotype 2 is assembled to scaffold level. The mitochondrial genome has also been assembled and is 17.44 kilobases in length.

References

Lopez-Garcia A, Gilabert J . Oral transmission of Chagas disease from a One Health approach: A systematic review. Trop Med Int Health. 2023; 28(9):689-698. DOI: 10.1111/tmi.13915. View

Rao S, Huntley M, Durand N, Stamenova E, Bochkov I, Robinson J . A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell. 2014; 159(7):1665-80. PMC: 5635824. DOI: 10.1016/j.cell.2014.11.021. View

Uliano-Silva M, R N Ferreira J, Krasheninnikova K, Formenti G, Abueg L, Torrance J . MitoHiFi: a python pipeline for mitochondrial genome assembly from PacBio high fidelity reads. BMC Bioinformatics. 2023; 24(1):288. PMC: 10354987. DOI: 10.1186/s12859-023-05385-y. View

Veiga Leprevost F, Gruning B, Alves Aflitos S, Rost H, Uszkoreit J, Barsnes H . BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017; 33(16):2580-2582. PMC: 5870671. DOI: 10.1093/bioinformatics/btx192. View

Danecek P, Bonfield J, Liddle J, Marshall J, Ohan V, Pollard M . Twelve years of SAMtools and BCFtools. Gigascience. 2021; 10(2). PMC: 7931819. DOI: 10.1093/gigascience/giab008. View

Ewels P, Magnusson M, Lundin S, Kaller M . MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016; 32(19):3047-8. PMC: 5039924. DOI: 10.1093/bioinformatics/btw354. View

Allio R, Schomaker-Bastos A, Romiguier J, Prosdocimi F, Nabholz B, Delsuc F . MitoFinder: Efficient automated large-scale extraction of mitogenomic data in target enrichment phylogenomics. Mol Ecol Resour. 2020; 20(4):892-905. PMC: 7497042. DOI: 10.1111/1755-0998.13160. View

Zhou C, McCarthy S, Durbin R . YaHS: yet another Hi-C scaffolding tool. Bioinformatics. 2022; 39(1). PMC: 9848053. DOI: 10.1093/bioinformatics/btac808. View

Abdennur N, Mirny L . Cooler: scalable storage for Hi-C data and other genomically labeled arrays. Bioinformatics. 2019; 36(1):311-316. PMC: 8205516. DOI: 10.1093/bioinformatics/btz540. View

10.

Vivas R, Garcia J, Guhl F, Hernandez C, Velasquez N, Ramirez J . Systematic review on the biology, ecology, genetic diversity and parasite transmission potential of Panstrongylus geniculatus (Latreille 1811) in Latin America. Mem Inst Oswaldo Cruz. 2021; 116:e200528. PMC: 7919531. DOI: 10.1590/0074-02760200528. View

11.

Quinlan A, Hall I . BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010; 26(6):841-2. PMC: 2832824. DOI: 10.1093/bioinformatics/btq033. View

12.

Tineo-Gonzalez E, Fermin R, Bonilla-Rivero A, Herrera L . Geographic Distribution of the Genus Berg, 1879 in the Neotropic with Emphasis on Vectors. Trop Med Infect Dis. 2023; 8(5). PMC: 10223185. DOI: 10.3390/tropicalmed8050272. View

13.

Crossa R, Hernandez M, Caraccio M, Rose V, Valente S, Valente V . Chromosomal evolution trends of the genus Panstrongylus (Hemiptera, Reduviidae), vectors of Chagas disease. Infect Genet Evol. 2003; 2(1):47-56. DOI: 10.1016/s1567-1348(02)00063-1. View

14.

Carrasco H, Torrellas A, Garcia C, Segovia M, Feliciangeli M . Risk of Trypanosoma cruzi I (Kinetoplastida: Trypanosomatidae) transmission by Panstrongylus geniculatus (Hemiptera: Reduviidae) in Caracas (Metropolitan District) and neighboring States, Venezuela. Int J Parasitol. 2005; 35(13):1379-84. DOI: 10.1016/j.ijpara.2005.05.003. View

15.

Malheiros Araujo Silvestrini M, Alessio G, Frias B, Ademar Sales Junior P, Araujo M, Silvestrini C . New insights into genetic diversity, and its influence on parasite biology and clinical outcomes. Front Immunol. 2024; 15:1342431. PMC: 11035809. DOI: 10.3389/fimmu.2024.1342431. View

16.

Formenti G, Abueg L, Brajuka A, Brajuka N, Gallardo-Alba C, Giani A . Gfastats: conversion, evaluation and manipulation of genome sequences using assembly graphs. Bioinformatics. 2022; 38(17):4214-4216. PMC: 9438950. DOI: 10.1093/bioinformatics/btac460. View

17.

Reyes-Lugo M, Rodriguez-Acosta A . Domiciliation of the sylvatic Chagas disease vector Panstrongylus geniculatus Latreille, 1811 (Triatominae: Reduviidae) in Venezuela. Trans R Soc Trop Med Hyg. 2001; 94(5):508. DOI: 10.1016/s0035-9203(00)90068-3. View

18.

Nakad Bechara C, Londono J, Segovia M, Leon Sanchez M, Martinez P C, Rodriguez R M . Genetic variability of Panstrongylus geniculatus (Reduviidae: Triatominae) in the Metropolitan District of Caracas, Venezuela. Infect Genet Evol. 2018; 66:236-244. DOI: 10.1016/j.meegid.2018.09.011. View

19.

. UniProt: the Universal Protein Knowledgebase in 2023. Nucleic Acids Res. 2022; 51(D1):D523-D531. PMC: 9825514. DOI: 10.1093/nar/gkac1052. View

20.

Arias-Giraldo L, Munoz M, Hernandez C, Herrera G, Velasquez-Ortiz N, Cantillo-Barraza O . Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing. Parasit Vectors. 2020; 13(1):434. PMC: 7457505. DOI: 10.1186/s13071-020-04310-z. View