Study of Resveratrol As an Antioxidant for Boar Semen Preservation: A Systematic Review

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2025 Mar 5

PMID 40041515

Authors

Ragil Angga Prastiya

Trilas Sardjito

Amung Logam Saputro

Sari Yanti Hayanti

Nining Haryuni

Samira Musa Sasi

Affiliations

Soon will be listed here.

Abstract

Background And Aim: In the global pig farming industry, artificial insemination (AI) is widely used to propagate high-quality genetics using processed semen that can be stored for extended periods. However, boar sperm are particularly susceptible to oxidative damage during storage and cryopreservation because of their high polyunsaturated fatty acid content. This study aimed to systematically review the impact of resveratrol (RVT), a potent antioxidant, on the preservation of boar semen.

Materials And Methods: A comprehensive meta-analysis was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Relevant articles were retrieved from databases such as Scopus, ScienceDirect, and PubMed using specific keywords. After a thorough screening, 10 studies were selected for inclusion. Data extracted from these studies included parameters such as sperm motility, viability, DNA integrity, and lipid peroxidation levels.

Results: Resveratrol supplementation in boar semen extenders significantly improved sperm quality under various storage conditions, including waterbath and cooled and frozen semen. RVT's antioxidative properties effectively reduced reactive oxygen species and prevented oxidative stress-related damage to sperm cells.

Conclusion: The addition of resveratrol to semen extenders enhances the preservation of boar sperm by mitigating oxidative damage, potentially increasing the success rate of AI in the swine industry. This study highlights the need for further research to optimize RVT dosage and application methods for different semen preservation techniques.

References

Shabani Nashtaei M, Nekoonam S, Naji M, Bakhshalizadeh S, Amidi F . Cryoprotective effect of resveratrol on DNA damage and crucial human sperm messenger RNAs, possibly through 5' AMP-activated protein kinase activation. Cell Tissue Bank. 2017; 19(1):87-95. DOI: 10.1007/s10561-017-9642-5. View

Kim S, Lee Y, Kim Y . Changes in sperm membrane and ROS following cryopreservation of liquid boar semen stored at 15 °C. Anim Reprod Sci. 2011; 124(1-2):118-24. DOI: 10.1016/j.anireprosci.2011.01.014. View

Torres M, Rigo V, Leal D, Pavaneli A, Muro B, Losano J . The use of resveratrol decreases liquid-extend boar semen fertility, even in concentrations that do not alter semen quality. Res Vet Sci. 2021; 136:360-368. DOI: 10.1016/j.rvsc.2021.03.011. View

Kaeoket K, Chanapiwat P . The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm. Animals (Basel). 2023; 13(18). PMC: 10526068. DOI: 10.3390/ani13182829. View

Gadani B, Bucci D, Spinaci M, Tamanini C, Galeati G . Resveratrol and Epigallocatechin-3-gallate addition to thawed boar sperm improves in vitro fertilization. Theriogenology. 2017; 90:88-93. DOI: 10.1016/j.theriogenology.2016.11.020. View

Serrano R, Garrido N, Cespedes J, Gonzalez-Fernandez L, Garcia-Marin L, Bragado M . Molecular Mechanisms Involved in the Impairment of Boar Sperm Motility by Peroxynitrite-Induced Nitrosative Stress. Int J Mol Sci. 2020; 21(4). PMC: 7072751. DOI: 10.3390/ijms21041208. View

Sun L, Wu C, Xu J, Zhang S, Dai J, Zhang D . Addition of butylated hydroxytoluene (BHT) in tris-based extender improves post-thaw quality and motion dynamics of dog spermatozoa. Cryobiology. 2020; 97:71-75. DOI: 10.1016/j.cryobiol.2020.10.006. View

Soares S, Brito C, Anciuti A, Gatti N, Corcini C, Varela Jr A . Nanocarried antioxidants in freezing extenders for boar spermatozoa. Andrologia. 2021; 53(10):e14199. DOI: 10.1111/and.14199. View

Cerolini S, Maldjian A, Surai P, Noble R . Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim Reprod Sci. 2000; 58(1-2):99-111. DOI: 10.1016/s0378-4320(99)00035-4. View

10.

Ammar O, Houas Z, Mehdi M . The association between iron, calcium, and oxidative stress in seminal plasma and sperm quality. Environ Sci Pollut Res Int. 2019; 26(14):14097-14105. DOI: 10.1007/s11356-019-04575-7. View

11.

Yang K, Wang N, Guo H, Wang J, Sun H, Sun L . Effect of L-carnitine on sperm quality during liquid storage of boar semen. Asian-Australas J Anim Sci. 2020; 33(11):1763-1769. PMC: 7649068. DOI: 10.5713/ajas.19.0455. View

12.

Altyeb Y, Absy G, Medan M, Hassan S, Elsayed D . The effects of cysteine and L-carnitine on the DNA integrity of post-thaw sperm of frozen buck semen. Iran J Vet Res. 2022; 23(3):196-203. PMC: 9681986. DOI: 10.22099/IJVR.2022.41489.6042. View

13.

Page M, McKenzie J, Bossuyt P, Boutron I, Hoffmann T, Mulrow C . The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ. 2021; 372:n71. PMC: 8005924. DOI: 10.1136/bmj.n71. View

14.

Feugang J, Rhoads C, Mustapha P, Tardif S, Parrish J, Willard S . Treatment of boar sperm with nanoparticles for improved fertility. Theriogenology. 2019; 137:75-81. DOI: 10.1016/j.theriogenology.2019.05.040. View

15.

Silva E, Cajueiro J, Silva S, Soares P, Guerra M . Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology. 2012; 77(8):1722-6. DOI: 10.1016/j.theriogenology.2011.11.023. View

16.

Bucak M, Ataman M, Baspinar N, Uysal O, Taspinar M, Bilgili A . Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andrologia. 2014; 47(5):545-52. DOI: 10.1111/and.12301. View

17.

Zhang X, Liu Q, Wang L, Yang G, Hu J . Effects of glutathione on sperm quality during liquid storage in boars. Anim Sci J. 2016; 87(10):1195-1201. DOI: 10.1111/asj.12545. View

18.

Hu Q . Effects of zinc chloride on boar sperm quality during liquid storage at 17°C. Vet Med Sci. 2023; 9(3):1217-1225. PMC: 10188062. DOI: 10.1002/vms3.1094. View

19.

Pervaiz S, Holme A . Resveratrol: its biologic targets and functional activity. Antioxid Redox Signal. 2009; 11(11):2851-97. DOI: 10.1089/ars.2008.2412. View

20.

Longobardi V, Salzano A, Campanile G, Marrone R, Palumbo F, Vitiello M . Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa. Theriogenology. 2016; 88:236-243. DOI: 10.1016/j.theriogenology.2016.09.031. View