Evaluation of Salivary Parameters and Remineralizing Effects of Yogurt in Counteracting the Cariogenic Impact of Candy Consumption: An in Vivo Study

Overview

Journal J Oral Biol Craniofac Res

Publisher Elsevier

Date 2025 Mar 3

PMID 40027859

Authors

Tanvi Shah

Kripa Dutta

Swagata Saha

Manju Raman Nair

Affiliations

Soon will be listed here.

Abstract

Background: The consumption of sugary snacks like candies alters the oral environment, increasing the risk of dental caries. This study evaluates changes in salivary pH, calcium, phosphorus, alkaline phosphatase, and total antioxidant capacity (TAC) following the consumption of candies and yogurt.

Aim: To compare the impact of candies on the oral environment and assess the effectiveness of rinsing with water versus consuming yogurt in reversing cariogenic changes in saliva.

Materials And Methods: Baseline saliva samples (5 mL) were collected from 60 children. Participants consumed candies, and saliva was collected 10 min post-consumption. They were divided into two groups: Group 1 rinsed with water, and Group 2 consumed yogurt. Saliva samples were collected at 10, 20 and 30 min post-intervention and analyzed for pH, calcium, phosphorus, alkaline phosphatase, and TAC. Data were analyzed using paired t-tests and post hoc Tukey tests.

Results: Candy consumption significantly decreased pH, calcium, phosphorus, and alkaline phosphatase levels (p < 0.001) and increased TAC levels (p < 0.001). Both water rinsing and yogurt consumption reversed these changes. Yogurt showed superior remineralization, with pH, calcium, phosphorus, and alkaline phosphatase levels returning to baseline more effectively than water rinsing (p < 0.001 for all comparisons). TAC levels were also restored more quickly with yogurt (p < 0.05).

Conclusion: Candy consumption induces a cariogenic environment. Both water rinsing and yogurt consumption effectively reversed these effects, with yogurt showing enhanced remineralization potential.

References

Jensdottir T, Nauntofte B, Buchwald C, Bardow A . Effects of calcium on the erosive potential of acidic candies in saliva. Caries Res. 2006; 41(1):68-73. DOI: 10.1159/000096108. View

Sionov R, Tsavdaridou D, Aqawi M, Zaks B, Steinberg D, Shalish M . Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans. BMC Oral Health. 2021; 21(1):136. PMC: 7980609. DOI: 10.1186/s12903-021-01502-6. View

Merritt J, Qi F, Shi W . Milk helps build strong teeth and promotes oral health. J Calif Dent Assoc. 2006; 34(5):361-6. View

Sonmez I, Aras S . Effect of white cheese and sugarless yoghurt on dental plaque acidogenicity. Caries Res. 2007; 41(3):208-11. DOI: 10.1159/000099320. View

Ferrazzano G, Cantile T, Quarto M, Ingenito A, Chianese L, Addeo F . Protective effect of yogurt extract on dental enamel demineralization in vitro. Aust Dent J. 2009; 53(4):314-9. DOI: 10.1111/j.1834-7819.2008.00072.x. View

Higham S, Edgar W . Effects of Parafilm and cheese chewing on human dental plaque pH and metabolism. Caries Res. 1989; 23(1):42-8. DOI: 10.1159/000261153. View

Malavalli P, Shetty S, Thimmaiah C, Ramlan A, Hugar S, Meharwade P . Evaluation of the Effect of Probiotic Yogurt Consumption on Salivary pH, Buffering Capacity and Calcium Level in 6-12-year-old Children: An Study. Int J Clin Pediatr Dent. 2023; 15(2):194-198. PMC: 10338946. DOI: 10.5005/jp-journals-10005-2368. View

Pandey R, Tripathi A, Chandra S, Pandey A . Relation of salivary phosphorus and alkaline phosphatase to the incidence of dental caries in children. J Pedod. 1990; 14(3):144-6. View

Enax J, Fandrich P, Schulze Zur Wiesche E, Epple M . The Remineralization of Enamel from Saliva: A Chemical Perspective. Dent J (Basel). 2024; 12(11). PMC: 11592461. DOI: 10.3390/dj12110339. View

10.

Baliga S, Muglikar S, Kale R . Salivary pH: A diagnostic biomarker. J Indian Soc Periodontol. 2013; 17(4):461-5. PMC: 3800408. DOI: 10.4103/0972-124X.118317. View

11.

Hokazono E, Osawa S, Nakano T, Kawamoto Y, Oguchi Y, Hotta T . Development of a new measurement method for serum calcium with chlorophosphonazo-III. Ann Clin Biochem. 2009; 46(Pt 4):296-301. DOI: 10.1258/acb.2009.008099. View

12.

Rugg-Gunn A, Edgar W, GEDDES D, JENKINS G . The effect of different meal patterns upon plaque pH in human subjects. Br Dent J. 1975; 139(9):351-6. DOI: 10.1038/sj.bdj.4803614. View

13.

Prieto P, Pineda M, Aguilar M . Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2):337-41. DOI: 10.1006/abio.1999.4019. View

14.

Touger-Decker R, van Loveren C . Sugars and dental caries. Am J Clin Nutr. 2003; 78(4):881S-892S. DOI: 10.1093/ajcn/78.4.881S. View

15.

Reynolds E . Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. J Dent Res. 1997; 76(9):1587-95. DOI: 10.1177/00220345970760091101. View

16.

Anil S, Anand P . Early Childhood Caries: Prevalence, Risk Factors, and Prevention. Front Pediatr. 2017; 5:157. PMC: 5514393. DOI: 10.3389/fped.2017.00157. View

17.

M Hegde A, Shetty R, Sequeira A . The acidogenicity of various chocolates available in Indian market: a comparative study. Int J Clin Pediatr Dent. 2014; 2(2):20-4. PMC: 4086558. DOI: 10.5005/jp-journals-10005-1025. View

18.

Ne Y, Frazao D, Bittencourt L, Fagundes N, Maranon-Vasquez G, Crespo-Lopez M . Are Dental Caries Associated with Oxidative Stress in Saliva in Children and Adolescents? A Systematic Review. Metabolites. 2022; 12(9). PMC: 9502212. DOI: 10.3390/metabo12090858. View

19.

Verakaki E, Duggal M . A comparison of different kinds of European chocolates on human plaque pH. Eur J Paediatr Dent. 2004; 4(4):203-10. View

20.

Varghese L, Mary Varughese J, Varghese N . Inhibitory effect of yogurt extract on dental enamel demineralisation - an in vitro study. Oral Health Prev Dent. 2013; 11(4):369-74. DOI: 10.3290/j.ohpd.a30604. View