Effects of Lamina-Chromatin Attachment on Super Long-Range Chromatin Interactions

Overview

Journal bioRxiv

Date 2025 Mar 3

PMID 40027763

Authors

Pourya Delafrouz

Hammad Farooq

Lin Du

Ao Ma

Jie Liang

Affiliations

Soon will be listed here.

Abstract

The interactions between chromatin and lamin proteins localized on the nuclear envelope play a crucial role in the three-dimensional (3D) organization of the genome. This study investigates the influence of lamin associated domains (LADs) on genome organization at the chromosome level using 3D polymer models of mouse embryonic fibroblasts (MEFs) and embryonic stem cells (mESCs). By integrating genome-wide LAD maps from DamID assays, we simulated chromatin conformations with and without LAD attachment to the nuclear envelope. Our results show that incorporating LAD-lamin interactions yields a radial chromatin distribution consistent with experimental observations. Moreover, LAD-lamin interactions induce significant super long-range chromatin contacts across distant genomic regions. These findings suggest two distinct mechanisms driving induction of chromatin interactions by LAD-lamin attachment.

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