Z/Ce@hemin Enzymes with Enhanced Peroxidase Activity for Monitoring and Screening the Oxidative Stress Models of Parkinson's Disease

Overview

Journal NPJ Parkinsons Dis

Date 2025 Mar 2

PMID 40025049

Authors

Li Lei

Xiuli Chen

Xincheng Long

Qiuxia Tu

Liping Zhao

Dan Huang

Lingli Song

Jiaojiao Wang

Suzhen Zhai

Xiaozhong Chen

Chunlin Zhang

Affiliations

Soon will be listed here.

Abstract

Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin onto zeolitic imidazolate framework-8 (ZIF-8)-encapsulated ceria (CeO) nanoparticles. This innovative design overcomes the challenges of hemin dimerization, enhances substrate affinity, and accelerates mass transport, leading to significantly improved peroxidase-like activity. The enzyme also demonstrates remarkable stability and resistance to environmental interference. Utilizing this Z/Ce@hemin composite, a sensitive and selective colorimetric detection system was developed for rapid and accurate quantification of hydrogen peroxide (HO), a key oxidative stress marker. This approach was successfully applied in cellular models and Caenorhabditis elegans models of Parkinson's disease, enabling precise monitoring of HO. The study provides a groundbreaking platform for oxidative stress modeling and expands possibilities in neurodegenerative disease research and therapeutic screening.

References

Brolin K, Bandres-Ciga S, Blauwendraat C, Widner H, Odin P, Hansson O . Insights on Genetic and Environmental Factors in Parkinson's Disease from a Regional Swedish Case-Control Cohort. J Parkinsons Dis. 2021; 12(1):153-171. PMC: 8842752. DOI: 10.3233/JPD-212818. View

Thirugnanam T, Santhakumar K . Chemically induced models of Parkinson's disease. Comp Biochem Physiol C Toxicol Pharmacol. 2021; 252:109213. DOI: 10.1016/j.cbpc.2021.109213. View

Jankovic J, Tan E . Parkinson's disease: etiopathogenesis and treatment. J Neurol Neurosurg Psychiatry. 2020; 91(8):795-808. DOI: 10.1136/jnnp-2019-322338. View

Koca F, Demirezen Yilmaz D, Onmaz N, Ocsoy I . Peroxidase-like activity and antimicrobial properties of curcumin-inorganic hybrid nanostructure. Saudi J Biol Sci. 2020; 27(10):2574-2579. PMC: 7499100. DOI: 10.1016/j.sjbs.2020.05.025. View

Chen R, Canales A, Anikeeva P . Neural Recording and Modulation Technologies. Nat Rev Mater. 2019; 2(2). PMC: 6707077. DOI: 10.1038/natrevmats.2016.93. View

Roussos A, Kitopoulou K, Borbolis F, Palikaras K . as a Model System to Study Human Neurodegenerative Disorders. Biomolecules. 2023; 13(3). PMC: 10046667. DOI: 10.3390/biom13030478. View

Lentz T, Burrage T, Smith A, Crick J, Tignor G . Is the acetylcholine receptor a rabies virus receptor?. Science. 1982; 215(4529):182-4. DOI: 10.1126/science.7053569. View

Zhang Q, Li M, Guo C, Jia Z, Wan G, Wang S . Fe₃O₄Nanoparticles Loaded on Lignin Nanoparticles Applied as a Peroxidase Mimic for the Sensitively Colorimetric Detection of H₂O₂. Nanomaterials (Basel). 2019; 9(2). PMC: 6410108. DOI: 10.3390/nano9020210. View

Connolly B, Lang A . Pharmacological treatment of Parkinson disease: a review. JAMA. 2014; 311(16):1670-83. DOI: 10.1001/jama.2014.3654. View

10.

Temur N, Dadi S, Nisari M, Ucuncuoglu N, Avan I, Ocsoy I . UV light promoted dihydrolipoic acid and its alanine derivative directed rapid synthesis of stable gold nanoparticles and their catalytic activity. Sci Rep. 2024; 14(1):24697. PMC: 11494073. DOI: 10.1038/s41598-024-76772-5. View

11.

Zhang W, Hu S, Yin J, He W, Lu W, Ma M . Prussian Blue Nanoparticles as Multienzyme Mimetics and Reactive Oxygen Species Scavengers. J Am Chem Soc. 2016; 138(18):5860-5. DOI: 10.1021/jacs.5b12070. View

12.

Pang S, Ho P, Liu H, Leung C, Li L, Chang E . The interplay of aging, genetics and environmental factors in the pathogenesis of Parkinson's disease. Transl Neurodegener. 2019; 8:23. PMC: 6696688. DOI: 10.1186/s40035-019-0165-9. View

13.

Xu J, Kao S, Lee F, Song W, Jin L, Yankner B . Dopamine-dependent neurotoxicity of alpha-synuclein: a mechanism for selective neurodegeneration in Parkinson disease. Nat Med. 2002; 8(6):600-6. DOI: 10.1038/nm0602-600. View

14.

Jiang B, Duan D, Gao L, Zhou M, Fan K, Tang Y . Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes. Nat Protoc. 2018; 13(7):1506-1520. DOI: 10.1038/s41596-018-0001-1. View

15.

Zhang P, Sun D, Cho A, Weon S, Lee S, Lee J . Modified carbon nitride nanozyme as bifunctional glucose oxidase-peroxidase for metal-free bioinspired cascade photocatalysis. Nat Commun. 2019; 10(1):940. PMC: 6391499. DOI: 10.1038/s41467-019-08731-y. View

16.

Dionisio P, Amaral J, Rodrigues C . Oxidative stress and regulated cell death in Parkinson's disease. Ageing Res Rev. 2021; 67:101263. DOI: 10.1016/j.arr.2021.101263. View

17.

Terstappen G, Meyer A, Bell R, Zhang W . Strategies for delivering therapeutics across the blood-brain barrier. Nat Rev Drug Discov. 2021; 20(5):362-383. DOI: 10.1038/s41573-021-00139-y. View

18.

Xiang S, Long X, Tu Q, Feng J, Zhang X, Feng G . Self-assembled, hemin-functionalized peptide nanotubes: an innovative strategy for detecting glutathione and glucose molecules with peroxidase-like activity. Nano Converg. 2023; 10(1):7. PMC: 9899300. DOI: 10.1186/s40580-023-00356-8. View

19.

Liu Q, Zhang L, Li H, Jia Q, Jiang Y, Yang Y . One-pot synthesis of porphyrin functionalized γ-Fe2O3 nanocomposites as peroxidase mimics for H2O2 and glucose detection. Mater Sci Eng C Mater Biol Appl. 2015; 55:193-200. DOI: 10.1016/j.msec.2015.05.028. View

20.

Poewe W, Seppi K, Tanner C, Halliday G, Brundin P, Volkmann J . Parkinson disease. Nat Rev Dis Primers. 2017; 3:17013. DOI: 10.1038/nrdp.2017.13. View