Assembly of a Synthetic Microbial Community to Ferment Rice (Oryza Sativa) Bran for Aquaculture Feedstuff

Overview

Journal Int Microbiol

Publisher Springer Nature

Specialty Microbiology

Date 2025 Feb 28

PMID 40019718

Authors

Antonio de Oliveira Vieira

Juliano De Dea Lindner

Adriano Faria Palmieri

Caio Francisco Santana Farias

Scheila Anelise Pereira Dutra

Ivan De Marco

Marco Shizuo Owatari

Mauricio Laterca Martins

Jose Luiz Pedreira Mourino

Affiliations

Soon will be listed here.

Abstract

Raw materials of plant origin, such as rice bran (Oryza sativa; RB), are promising alternatives to fishmeal in aquaculture feeds, offering a low-cost solution. However, due to antinutritional factors and reduced digestibility, direct use of RB is limited. Fermentation is an effective, cost-effective, and environmentally friendly technique that improves the nutritional quality of RB, enhancing nutrient availability and digestibility and reducing harmful compounds. Fermented RB improved growth, feed utilization, immune competence, and gut health, contributing to more sustainable aquaculture practices. The study aimed to evaluate the solid-state fermentation influence of a synthetic microbial community (SynCom) on RB (RBMC). The fermentation by the microbial consortium showed significant changes in RB physical-chemical composition and crude fiber and protein. Significant reductions were observed for ether extract, mineral matter, phosphate, phosphorus, and potassium compared with the naturally fermented RB (RBNF). Sodium, calcium, and iron contents increased by 43.03, 60.77, and 74.58%, respectively, compared to RBNF. A significant increase was observed in the fermented RBMC for essential and non-essential amino acids. Scanning electron microscopy revealed changes in the microstructure of the RB, in addition to the presence of microbial aggregates morphologically similar to the individuals used as inoculum. The RB fermentation using SynCom significantly improved the quality of the RB by-product feedstuff. The use of fermented RB in diet formulations for aquatic organisms is desirable because it enables the reuse of this industrial co-product, which is rich in nutrients and biological value.

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