Effects of Epicatechin on the Expression of MyomiRs-31, -133, -136, -206, -296, and -486 in the Skeletal Muscle of the Offspring of Obese Mothers

Overview

Journal Cell Biochem Biophys

Specialties Biochemistry
Biophysics
Cell Biology

Date 2025 Feb 27

PMID 40016564

Authors

Paola B Zarate-Segura

Ana Luisa Alvarez-Chavez

Sergio De Los Santos

Fernando G Bastida-Gonzalez

Jose Manuel Hernandez-Hernandez

Elena Zambrano

Ramon Mauricio Coral-Vazquez

Patricia Canto

Affiliations

Soon will be listed here.

Abstract

Specific myogenic microRNAs termed "myomiRNAs" are involved in skeletal muscle development and regeneration, and an obesogenic environment in utero may affect these processes. The present study aimed to determine whether this environment induced variations in the expression levels of myomiRs-31, -133, -136, -206, and -296 and whether the administration of (-)-epicatechin (Epi), an exercise mimetic, could modify these variations. Rat Wistar male offspring from control mothers (C) or obese mothers (MO) were treated (C+Epi and MO+Epi) or not treated with Epi (C and MO). MyomiRNA expression in the gastrocnemius and soleus muscles was analyzed via RT‒qPCR, and bioinformatic analysis was used to predict the participation of these miRNAs in different skeletal muscle signal transduction pathways. The expression of myomiRNA-31-5p in the gastrocnemius and soleus was significantly lower in the Epi-treated groups (C+Epi and MO+Epi vs. C and MO). The expression of myomiRNA-206 increased in the gastrocnemius muscles of the MO and MO+Epi groups but decreased in the soleus muscles of the MO and MO+Epi groups. The expression of myomiRNA-296 was increased in the MO group in the gastrocnemius and soleus but was reduced in the Epi stimulus group. The expression of myomiRNA-486 increased in the gastrocnemius of the C+Epi group and decreased in the soleus of the MO+Epi group (p = 0.028 vs. MO). In conclusion, we show that an intrauterine obesogenic environment differentially affects the expression levels of some myomiRNAs and that this effect is modified by epicatechin.

References

Zambrano E, Ibanez C, Martinez-Samayoa P, Lomas-Soria C, Durand-Carbajal M, Rodriguez-Gonzalez G . Maternal Obesity: Lifelong Metabolic Outcomes for Offspring from Poor Developmental Trajectories During the Perinatal Period. Arch Med Res. 2016; 47(1):1-12. DOI: 10.1016/j.arcmed.2016.01.004. View

Godfrey K, Reynolds R, Prescott S, Nyirenda M, Jaddoe V, Eriksson J . Influence of maternal obesity on the long-term health of offspring. Lancet Diabetes Endocrinol. 2016; 5(1):53-64. PMC: 5245733. DOI: 10.1016/S2213-8587(16)30107-3. View

Dearden L, Ozanne S . Early life impacts of maternal obesity: a window of opportunity to improve the health of two generations. Philos Trans R Soc Lond B Biol Sci. 2023; 378(1885):20220222. PMC: 10363703. DOI: 10.1098/rstb.2022.0222. View

Buckingham M . Skeletal muscle formation in vertebrates. Curr Opin Genet Dev. 2001; 11(4):440-8. DOI: 10.1016/s0959-437x(00)00215-x. View

Perry R, Rudnick M . Molecular mechanisms regulating myogenic determination and differentiation. Front Biosci. 2000; 5:D750-67. DOI: 10.2741/perry. View

Bayol S, Simbi B, Stickland N . A maternal cafeteria diet during gestation and lactation promotes adiposity and impairs skeletal muscle development and metabolism in rat offspring at weaning. J Physiol. 2005; 567(Pt 3):951-61. PMC: 1474235. DOI: 10.1113/jphysiol.2005.088989. View

Mikovic J, Brightwell C, Lindsay A, Wen Y, Kowalski G, Russell A . An obesogenic maternal environment impairs mouse growth patterns, satellite cell activation, and markers of postnatal myogenesis. Am J Physiol Endocrinol Metab. 2020; 319(6):E1008-E1018. DOI: 10.1152/ajpendo.00398.2020. View

ORourke J, Georges S, Seay H, Tapscott S, McManus M, Goldhamer D . Essential role for Dicer during skeletal muscle development. Dev Biol. 2007; 311(2):359-68. PMC: 2753295. DOI: 10.1016/j.ydbio.2007.08.032. View

Horak M, Novak J, Bienertova-Vasku J . Muscle-specific microRNAs in skeletal muscle development. Dev Biol. 2015; 410(1):1-13. DOI: 10.1016/j.ydbio.2015.12.013. View

10.

Jing Y, Gan M, Xie Z, Ma J, Chen L, Zhang S . Characteristics of microRNAs in Skeletal Muscle of Intrauterine Growth-Restricted Pigs. Genes (Basel). 2023; 14(7). PMC: 10379088. DOI: 10.3390/genes14071372. View

11.

Harnly J, Doherty R, Beecher G, Holden J, Haytowitz D, Bhagwat S . Flavonoid content of U.S. fruits, vegetables, and nuts. J Agric Food Chem. 2006; 54(26):9966-77. DOI: 10.1021/jf061478a. View

12.

Ramirez-Sanchez I, De Los Santos S, Gonzalez-Basurto S, Canto P, Mendoza-Lorenzo P, Palma-Flores C . (-)-Epicatechin improves mitochondrial-related protein levels and ameliorates oxidative stress in dystrophic δ-sarcoglycan null mouse striated muscle. FEBS J. 2014; 281(24):5567-80. PMC: 4262719. DOI: 10.1111/febs.13098. View

13.

McDonald C, Ramirez-Sanchez I, Oskarsson B, Joyce N, Aguilar C, Nicorici A . (-)-Epicatechin induces mitochondrial biogenesis and markers of muscle regeneration in adults with Becker muscular dystrophy. Muscle Nerve. 2020; 63(2):239-249. PMC: 7898288. DOI: 10.1002/mus.27108. View

14.

Ramirez-Ramirez M, Fernandez-Valverde F, Resendiz-Garcia A, Martinez-Damas M, Cano-Martinez L, Zentella-Dehesa A . (-) - Epicatechin improves Tibialis anterior muscle repair in CD1 mice with BaCl-induced damage. J Nutr Biochem. 2022; 107:109069. DOI: 10.1016/j.jnutbio.2022.109069. View

15.

Munguia L, Ramirez-Sanchez I, Meaney E, Villarreal F, Ceballos G, Najera N . Flavonoids from dark chocolate and (-)-epicatechin ameliorate high-fat diet-induced decreases in mobility and muscle damage in aging mice. Food Biosci. 2020; 37. PMC: 7497789. DOI: 10.1016/j.fbio.2020.100710. View

16.

Samani A, Hightower R, Reid A, English K, Lopez M, Doyle J . miR-486 is essential for muscle function and suppresses a dystrophic transcriptome. Life Sci Alliance. 2022; 5(9). PMC: 9087951. DOI: 10.26508/lsa.202101215. View

17.

Liu N, Williams A, Maxeiner J, Bezprozvannaya S, Shelton J, Richardson J . microRNA-206 promotes skeletal muscle regeneration and delays progression of Duchenne muscular dystrophy in mice. J Clin Invest. 2012; 122(6):2054-65. PMC: 3366415. DOI: 10.1172/JCI62656. View

18.

Dmitriev P, Barat A, Polesskaya A, OConnell M, Robert T, Dessen P . Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes. BMC Genomics. 2013; 14:265. PMC: 3639941. DOI: 10.1186/1471-2164-14-265. View

19.

Gentile A, Lhamyani S, Coin-Araguez L, Clemente-Postigo M, Olivera W, Romero-Zerbo S . miR-20b, miR-296, and Let-7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes. Obesity (Silver Spring). 2019; 27(2):245-254. DOI: 10.1002/oby.22363. View

20.

Guerrieri D, Moon H, van Praag H . Exercise in a Pill: The Latest on Exercise-Mimetics. Brain Plast. 2018; 2(2):153-169. PMC: 5928571. DOI: 10.3233/BPL-160043. View