Dietary and Environmental Factors Affecting the Dynamics of the Gut Bacteria in Tibetan Awang Sheep () Across Divergent Breeding Models

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2025 Feb 26

PMID 40008045

Authors

Yuhao Wang

Xinping Wang

Yirong Wang

Songyu Liao

Zhaxi Pubu

Jiangcuo Silang

Lixu Chai

Siyue Zhao

Affiliations

Soon will be listed here.

Abstract

Introduction: Tibetan Awang sheep (), indigenous to the Qinghai-Tibet Plateau, are highly adapted to high-altitude environment. However, knowledge regarding their gut bacterial composition remains limited.

Methods: A comprehensive 16S rRNA highthroughput sequencing was performed on fecal samples from 15 Awang sheep under pure grazing, semi-captivity, and full captivity breeding models.

Results: Our results revealed that Firmicutes and Bacteroidetes were the most abundant bacterial phyla, while , , , , and were prevalent genera in the gut microbiota of Awang sheep. Meanwhile, the predominant presence of with increasing altitude of breeding locations indirectly demonstrates its crucial role in mediating energy acquisition among Awang sheep at high altitudes. Furthermore, PCoA and ANOSIM analysis exhibited significant differences in bacterial composition across all breeding models ( > 0.6, < 0.001). , , and were significantly abundant in the pure grazing breeding model, while and were more abundant in the semi-captivity breeding model. An abnormally high abundance of indicated a potential risk of infection in the fully captive group. The environmental association analysis exhibited that meadows diet ( = 0.938, Pr[>r] = 0.001) and altitude ( = 0.892, Pr[>r] = 0.001) had significant effects on the dominant genera, explaining a substantial proportion of the total variation in community composition.

Discussion: Our study indicated that breeding conditions significantly impact the gut microbiota of Awang sheep. The environmental association analysis underscores the importance of diet and altitude in shaping the gut microbiota of Awang sheep. The present findings provide insights into the microbiota dynamics of Awang sheep and offer guidance for their scientific husbandry management.

References

Pang K, Dai D, Yang Y, Wang X, Liu S, Huang W . Effects of high concentrate rations on ruminal fermentation and microbiota of yaks. Front Microbiol. 2022; 13:957152. PMC: 9558216. DOI: 10.3389/fmicb.2022.957152. View

Henderson G, Cox F, Ganesh S, Jonker A, Young W, Janssen P . Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep. 2015; 5:14567. PMC: 4598811. DOI: 10.1038/srep14567. View

Li Y, Hu X, Yang S, Zhou J, Zhang T, Qi L . Comparative Analysis of the Gut Microbiota Composition between Captive and Wild Forest Musk Deer. Front Microbiol. 2017; 8:1705. PMC: 5591822. DOI: 10.3389/fmicb.2017.01705. View

Thapa S, Zhou S, OHair J, Al Nasr K, Ropelewski A, Li H . Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach. BMC Biotechnol. 2023; 23(1):51. PMC: 10696843. DOI: 10.1186/s12896-023-00821-6. View

Ma Y, Deng X, Yang X, Wang J, Li T, Hua G . Characteristics of Bacterial Microbiota in Different Intestinal Segments of Aohan Fine-Wool Sheep. Front Microbiol. 2022; 13:874536. PMC: 9097873. DOI: 10.3389/fmicb.2022.874536. View

Douglas G, Maffei V, Zaneveld J, Yurgel S, Brown J, Taylor C . PICRUSt2 for prediction of metagenome functions. Nat Biotechnol. 2020; 38(6):685-688. PMC: 7365738. DOI: 10.1038/s41587-020-0548-6. View

Liu J, Lu Z, Yuan C, Wang F, Yang B . Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on Sequences. Animals (Basel). 2020; 10(7). PMC: 7401538. DOI: 10.3390/ani10071177. View

Wang X, Zhang Z, Wang X, Bao Q, Wang R, Duan Z . The Impact of Host Genotype, Intestinal Sites and Probiotics Supplementation on the Gut Microbiota Composition and Diversity in Sheep. Biology (Basel). 2021; 10(8). PMC: 8389637. DOI: 10.3390/biology10080769. View

Zhang Y, Choi S, Nogoy K, Liang S . Review: The development of the gastrointestinal tract microbiota and intervention in neonatal ruminants. Animal. 2021; 15(8):100316. DOI: 10.1016/j.animal.2021.100316. View

10.

Wang Y, Xu B, Chen H, Yang F, Huang J, Jiao X . Environmental factors and gut microbiota: Toward better conservation of deer species. Front Microbiol. 2023; 14:1136413. PMC: 10027939. DOI: 10.3389/fmicb.2023.1136413. View

11.

Couch C, Arnold H, Crowhurst R, Jolles A, Sharpton T, Witczak M . Bighorn sheep gut microbiomes associate with genetic and spatial structure across a metapopulation. Sci Rep. 2020; 10(1):6582. PMC: 7171152. DOI: 10.1038/s41598-020-63401-0. View

12.

Guo R, Zhang W, Shen W, Zhang G, Xie T, Li L . Analysis of gut microbiota in chinese donkey in different regions using metagenomic sequencing. BMC Genomics. 2023; 24(1):524. PMC: 10478257. DOI: 10.1186/s12864-023-09575-z. View

13.

Wanjala G, Astuti P, Bagi Z, Kichamu N, Strausz P, Kusza S . A review on the potential effects of environmental and economic factors on sheep genetic diversity: Consequences of climate change. Saudi J Biol Sci. 2022; 30(1):103505. PMC: 9718971. DOI: 10.1016/j.sjbs.2022.103505. View

14.

Liu D, Gao X, Huang X, Fan Y, Wang Y, Zhang Y . Moderate altitude exposure impacts host fasting blood glucose and serum metabolome by regulation of the intestinal flora. Sci Total Environ. 2023; 905:167016. DOI: 10.1016/j.scitotenv.2023.167016. View

15.

Martinson V, Douglas A, Jaenike J . Community structure of the gut microbiota in sympatric species of wild Drosophila. Ecol Lett. 2017; 20(5):629-639. DOI: 10.1111/ele.12761. View

16.

Jiang X, Peng X, Deng G, Sheng H, Wang Y, Zhou H . Illumina sequencing of 16S rRNA tag revealed spatial variations of bacterial communities in a mangrove wetland. Microb Ecol. 2013; 66(1):96-104. DOI: 10.1007/s00248-013-0238-8. View

17.

Li J, Sun L, He X, Liu J, Wang D, Han Y . Succession of the Gut Microbiome in the Tibetan Population of Minjiang River Basin. Front Microbiol. 2022; 13:834335. PMC: 9035803. DOI: 10.3389/fmicb.2022.834335. View

18.

Lv W, Liu X, Sha Y, Shi H, Wei H, Luo Y . Rumen Fermentation-Microbiota-Host Gene Expression Interactions to Reveal the Adaptability of Tibetan Sheep in Different Periods. Animals (Basel). 2021; 11(12). PMC: 8697948. DOI: 10.3390/ani11123529. View

19.

Zhao S, Li C, Zhu T, Jin L, Deng W, Zhao K . Diversity and Composition of Gut Bacterial Community in Giant Panda with Anorexia. Curr Microbiol. 2021; 78(4):1358-1366. DOI: 10.1007/s00284-021-02424-w. View

20.

Wang X, Zhang Z, Li B, Hao W, Yin W, Ai S . Depicting Fecal Microbiota Characteristic in Yak, Cattle, Yak-Cattle Hybrid and Tibetan Sheep in Different Eco-Regions of Qinghai-Tibetan Plateau. Microbiol Spectr. 2022; 10(4):e0002122. PMC: 9430443. DOI: 10.1128/spectrum.00021-22. View