Deciphering Viral Replication Dynamics in Feline Infectious Peritonitis: A Quantitative Approach

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2025 Feb 26

PMID 40007034

Authors

Carole J Burgener

Emi N Barker

Teodoru Soare

Diana-Gabriela Soare

Andrea M Spiri

Alexandra J Malbon

Marina L Meli

Anja Kipar

Affiliations

Soon will be listed here.

Abstract

Feline infectious peritonitis (FIP) is a complex immune-mediated disease caused by feline coronavirus (FCoV). Despite advancements in understanding its pathogenesis, challenges persist in elucidating viral factors related to virion composition and replication. This study examined FCoV-infected cats with and without FIP for potential associations with or variation in expression ratios for different viral genes. We analyzed tissue samples with FIP lesions from 46 cats with FIP and mesenteric lymph nodes from 10 FCoV-infected cats (7b RT-qPCR positive) without FIP with three RT-qPCR assays, targeting (sub)-genomic RNAs of the RNA-dependent RNA polymerase (RdRp) and envelope (E) genes. In cats with FIP, the RdRp mRNA assay yielded the highest copy numbers, followed by the combined RdRp gRNA and mRNA assay; the E mRNA assay yielded the lowest copy numbers. In cats without FIP, significantly fewer viral RNA copies were detected regardless of the assay. Viral gene expression was not detected in six and was observed only at low levels in one or more assays in four samples. The observed correlation between assays and the intragroup correlation assay indicate consistent transcription of both the structural E protein and RdRp genes within FIP lesions in cats with FIP but not in cats with systemic FCoV infection alone.

References

Emmler L, Felten S, Matiasek K, Balzer H, Pantchev N, Leutenegger C . Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis. J Feline Med Surg. 2019; 22(8):791-799. PMC: 7206566. DOI: 10.1177/1098612X19886671. View

BOS E, Luytjes W, van der Meulen H, Koerten H, Spaan W . The production of recombinant infectious DI-particles of a murine coronavirus in the absence of helper virus. Virology. 1996; 218(1):52-60. PMC: 7130547. DOI: 10.1006/viro.1996.0165. View

Ruch T, Machamer C . The coronavirus E protein: assembly and beyond. Viruses. 2012; 4(3):363-82. PMC: 3347032. DOI: 10.3390/v4030363. View

Simons F, Vennema H, Rofina J, Pol J, Horzinek M, Rottier P . A mRNA PCR for the diagnosis of feline infectious peritonitis. J Virol Methods. 2005; 124(1-2):111-6. PMC: 7112896. DOI: 10.1016/j.jviromet.2004.11.012. View

Pesteanu-Somogyi L, Radzai C, Pressler B . Prevalence of feline infectious peritonitis in specific cat breeds. J Feline Med Surg. 2005; 8(1):1-5. PMC: 7128820. DOI: 10.1016/j.jfms.2005.04.003. View

Lim K, Liu D . The missing link in coronavirus assembly. Retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins. J Biol Chem. 2001; 276(20):17515-23. PMC: 7982318. DOI: 10.1074/jbc.M009731200. View

Kipar A, BELLMANN S, Kremendahl J, Kohler K, Reinacher M . Cellular composition, coronavirus antigen expression and production of specific antibodies in lesions in feline infectious peritonitis. Vet Immunol Immunopathol. 1998; 65(2-4):243-57. PMC: 7119884. DOI: 10.1016/s0165-2427(98)00158-5. View

Gunn-Moore D, Gruffydd-Jones T, Harbour D . Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis. Vet Microbiol. 1998; 62(3):193-205. PMC: 7117229. DOI: 10.1016/s0378-1135(98)00210-7. View

Weiss R, Scott F . Pathogenesis of feline infetious peritonitis: pathologic changes and immunofluorescence. Am J Vet Res. 1981; 42(12):2036-48. View

10.

Gut M, Leutenegger C, Huder J, Pedersen N, Lutz H . One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses. J Virol Methods. 1999; 77(1):37-46. PMC: 7185542. DOI: 10.1016/s0166-0934(98)00129-3. View

11.

Malbon A, Meli M, Barker E, Davidson A, Tasker S, Kipar A . Inflammatory Mediators in the Mesenteric Lymph Nodes, Site of a Possible Intermediate Phase in the Immune Response to Feline Coronavirus and the Pathogenesis of Feline Infectious Peritonitis?. J Comp Pathol. 2019; 166:69-86. PMC: 7094650. DOI: 10.1016/j.jcpa.2018.11.001. View

12.

Corse E, Machamer C . The cytoplasmic tails of infectious bronchitis virus E and M proteins mediate their interaction. Virology. 2003; 312(1):25-34. PMC: 7127533. DOI: 10.1016/s0042-6822(03)00175-2. View

13.

Pedersen N . Virologic and immunologic aspects of feline infectious peritonitis virus infection. Adv Exp Med Biol. 1987; 218:529-50. DOI: 10.1007/978-1-4684-1280-2_69. View

14.

Fehr A, Perlman S . Coronaviruses: an overview of their replication and pathogenesis. Methods Mol Biol. 2015; 1282:1-23. PMC: 4369385. DOI: 10.1007/978-1-4939-2438-7_1. View

15.

Vkovski P, Kratzel A, Steiner S, Stalder H, Thiel V . Coronavirus biology and replication: implications for SARS-CoV-2. Nat Rev Microbiol. 2020; 19(3):155-170. PMC: 7592455. DOI: 10.1038/s41579-020-00468-6. View

16.

Meli M, Kipar A, Muller C, Jenal K, Gonczi E, Borel N . High viral loads despite absence of clinical and pathological findings in cats experimentally infected with feline coronavirus (FCoV) type I and in naturally FCoV-infected cats. J Feline Med Surg. 2004; 6(2):69-81. PMC: 7128724. DOI: 10.1016/j.jfms.2003.08.007. View

17.

Klein-Richers U, Hartmann K, Hofmann-Lehmann R, Unterer S, Bergmann M, Rieger A . Prevalence of Feline Coronavirus Shedding in German Catteries and Associated Risk Factors. Viruses. 2020; 12(9). PMC: 7551668. DOI: 10.3390/v12091000. View

18.

Felten S, Klein-Richers U, Hofmann-Lehmann R, Bergmann M, Unterer S, Leutenegger C . Correlation of Feline Coronavirus Shedding in Feces with Coronavirus Antibody Titer. Pathogens. 2020; 9(8). PMC: 7459802. DOI: 10.3390/pathogens9080598. View

19.

Kipar A, Meli M, Failing K, Euler T, Gomes-Keller M, Schwartz D . Natural feline coronavirus infection: differences in cytokine patterns in association with the outcome of infection. Vet Immunol Immunopathol. 2006; 112(3-4):141-55. PMC: 7112699. DOI: 10.1016/j.vetimm.2006.02.004. View

20.

Vennema H, Godeke G, Rossen J, Voorhout W, Horzinek M, Opstelten D . Nucleocapsid-independent assembly of coronavirus-like particles by co-expression of viral envelope protein genes. EMBO J. 1996; 15(8):2020-8. PMC: 450121. View